Gelred nucleic acid gel stain

Manufactured by GoldBio

Sourced in United States

GelRed™ is a nucleic acid gel stain used for the detection of DNA and RNA in agarose or polyacrylamide gels. It binds to nucleic acids and emits fluorescence when exposed to UV or blue light, allowing for the visualization of DNA and RNA bands.

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Lab products found in correlation

Smartspec plus spectrophotometer, by Bio-Rad (1 mentions) Zetasizer nano, by Malvern Panalytical (1 mentions) Pierce bca assay kit, by Thermo Fisher Scientific (1 mentions) Nupage 12 bis tris protein gel, by Thermo Fisher Scientific (1 mentions) Akta fplc 900 system, by GE Healthcare (1 mentions) Superose 6 increase 10 300 gl column, by GE Healthcare (1 mentions) Tecnai g2 spirit biotwin transmission electron microscope, by Thermo Fisher Scientific (1 mentions) Coomassie brilliant blue g 250, by Merck Group (1 mentions)

4 protocols using gelred nucleic acid gel stain

Soil Microbial Community Analysis via DGGE

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DNA was extracted from the soil samples and stored at −20°C using the E.Z.N.A. Soil DNA Kit (Omega, United States) following the protocol of the manufacturer. The purity and concentration of DNA were measured by a SmartSpec^™ Plus spectrophotometer (Bio-Rad, United States), and the quality was checked by agarose gel electrophoresis (Invitrogen, United States) with the GelRed^™ nucleic acid gel stain (Goldbio, United States). We pooled three DNA samples from different replicates of the same treatments after DNA extraction for DGGE. Nested PCR was used to increase the resolution yield of DGGE. The highly variable V3 region of bacterial 16S rRNA gene was amplified as described by Xu et al. (2012) (link) with a GC clamp (40 bp adhere to the 5' end) incorporated at the 5' end in primer 341F. The ITS1 region of the fungal 18S rRNA gene was amplified as described by Yu et al. (2013) (link) with GC clamp (40 bp adhere to the 5' end) in primer ITS1-f. The second round of PCR yielded amplicons of bacteria and fungi that were approximately 190 bp and 250 bp, respectively. The primers used in this study can be found in Supplementary Table 1.

Chu H., Wang H., Zhang Y., Li Z., Wang C., Dai D, & Tang M. (2021). Inoculation With Ectomycorrhizal Fungi and Dark Septate Endophytes Contributes to the Resistance of Pinus spp. to Pine Wilt Disease. Frontiers in Microbiology, 12, 687304.

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Characterization of Qβ-based VLPs

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The Qβ-based VLPs (Qβ, QβApoB, QβCETP, and QβPCSK9) were characterized as previously described^{[29 (link)]} using fast protein liquid chromatography (FPLC), transmission electron microscopy (TEM), dynamic light scattering (DLS), sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), and agarose gel electrophoresis. The Qβ VLPs concentration was determined by measuring the total protein using a Pierce BCA assay kit (Thermo Fisher Scientific). FPLC was performed using an AKTA-FPLC 900 system fitted with Superose 6 Increase 10/300 GL columns (GE Healthcare) using PBS as the mobile phase at flow rate 0.5 mL/min. TEM images were acquired on a FEI Tecnai Spirit G2 Bio TWIN transmission electron microscope. Samples were mounted on 400-mesh hexagonal copper grids and stained with 2% (v/v) uranyl acetate. DLS was carried out on a Malvern Instruments Zetasizer Nano at 25 °C in plastic disposal cuvettes. SDS-PAGE was performed under reducing conditions on NuPAGE 12% Bis-Tris protein gels [Thermo Fisher Scientific] at 120 mV for 35 min. and stained with Coomassie Brilliant Blue. Agarose gels (0.8% (w/v) in TAE buffer) were pre-stained with Gelred™ Nucleic Acid Gel Stain [GoldBio] and samples ran under non-reducing conditions at 100 mV for 30min. The gel images were acquired using the ProteinSimple FluorChem R imaging system.

Ortega-Rivera O.A., Pokorski J.K, & Steinmetz N.F. (2021). A single-dose, implant-based, trivalent virus-like particle vaccine against “cholesterol checkpoint” proteins. Advanced therapeutics, 4(8), 2100014.

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Agarose Gel Electrophoresis of CPMV

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1.2% (w/v) agarose gels were run for 30 min at 120 V and 400 mA in 1× tris‐acetate‐EDTA (TAE) running buffer in the presence of GelRed Nucleic Acid Gel Stain (GoldBio) diluted 1:10,000 (v/v). 10 μg of CPMV formulations were prepared in 0.1 M KP along with 6× Gel Loading Purple dye (Biolabs) and analyzed in sample wells. Gels were imaged before and after staining with 0.25% (w/v) Coomassie Brilliant Blue G‐250 from Sigma Aldrich (St Louis, MO, USA) using the ProteinSimple FluorChem R imaging system (MN, USA) under UV light and white light, respectively.

Shin M.D., Jung E., Moreno‐Gonzalez M.A., Ortega‐Rivera O.A, & Steinmetz N.F. (2023). Pluronic F127 “nanoarmor” for stabilization of Cowpea mosaic virus immunotherapy. Bioengineering & Translational Medicine, 9(1), e10574.

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CPMV RNA and Protein Visualization

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The native and S100A9-targeted CPMV samples were diluted to a final concentration of 10 μg in 30 μL and run through a 1.2% (w/v) agarose gel stained with GelRed nucleic acid gel stain (Gold Biotechnology). The parameters were set at 120 V and 400 mA for 30 min. The RNA within the CPMV was imaged using the AlphaImager System, and the gel was then stained overnight in 0.25% (w/v) Coomassie Blue followed by destaining in a solution of deionized water, methanol, and acetic acid in a 50:40:10 (v:v:v) ratio. The resulting protein bands were imaged using the AlphaImager System.

Chung Y.H., Volckaert B.A, & Steinmetz N.F. (2022). Metastatic Colon Cancer Treatment Using S100A9-Targeted Cowpea Mosaic Virus Nanoparticles. Biomacromolecules, 23(12), 5127-5136.

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