Hiscript q select rt supermix kit

Total RNA was extracted from harvested GCO cells using Trizol (Accurate Biology Co. Ltd., Shenzhen, China) according to the manufacturer’s protocol. The quality and the purity of isolated RNA were determined using a Nanodrop 2000c spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA was reverse-transcribed to cDNA using the HiScript Q Select RT Supermix kit (Vazyme, Nanjing, China) for qPCR (+gDNA wiper). The qRT-PCR protocol was as follows: 95 °C for 4 min, and then 38 cycles at 95 °C denaturation for 10 s, followed by annealing at 59 °C for 30 s with a Bio-Rad iCycler IQ5 Multicolor real time PCR detection system. Table 1 shows the primers used for qRT-PCR in the study.

Total RNA of various rice tissues was extracted using the E.Z.N.A.^® Plant RNA Kit (Omega Bio-tek, Norcross, GA, USA) and reverse transcribed using the HiScript^® Q Select RT SuperMix kit (Vazyme, Nanjing, China) in accordance with the manufacturer’s instructions. qPCR was performed by using the AceQ qPCR SYBR Green Master Mix (Vazyme, Nanjing, China) with the corresponding primer sets on a Real-Time PCR System (Bio-Rad, CFX96, Hercules, CA, USA). OsACTIN1 was used as the normalized reference gene. The relative levels of gene expression were calculated as described previously [84 (link)].