Mcherry cortactin

Manufactured by Addgene

mCherry-cortactin is a fluorescently labeled protein construct that consists of the mCherry fluorescent protein fused to the cortactin protein. Cortactin is a cytoskeletal protein involved in the regulation of actin dynamics. The mCherry fluorescent tag allows for the visualization and tracking of cortactin localization within cells.

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Lab products found in correlation

Arp3 mcherry, by Addgene (2 mentions) Memerald lifeact, by Addgene (2 mentions) Cmv gfp nmhc 2 a, by Addgene (2 mentions)

2 protocols using mcherry cortactin

Fluorescently Tagged Podosome Proteins

Cited 1 time

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Human myo1e and myo1f constructs tagged with EGFP have been previously described (Barger et al., 2019 (link)). mEmerald-Lifeact was a gift from Michael Davidson (Addgene #54148). Cofilin-EGFP was a gift from James Bamburg (Addgene #50859). Chicken regulatory light chain (RLC) tagged with EGFP was a gift from Klaus Hahn. WASP tagged with myc was a gift from Dianne Cox, and was subcloned into a pUB-Halo-C1 vector. CMV-GFP-NMHCII-A was a gift from Robert Adelstein (Addgene #11347). ARP3-mCherry (Addgene #27682) and mCherry-cortactin (Addgene #27676) were gifts from Christien Merrifield that were subcloned into EGFP-C1 and mEmerald-C1, respectively. Chicken paxillin was a gift from Chris Turner, which was subcloned into mScarlet-i-C1. Chicken vinculin was a gift from Kenneth Yamada (Addgene #50513) and subcloned into pUB-mEmerald-C1. Immunohistochemical staining to determine localization of select podosome-related proteins in relation to the actin teeth did not produce good results, which may be due to the adhesive and porous nature of the IgG-functionalized DAAMPs. As an alternative, we transfected the RAW macrophages with fluorescently tagged proteins. All transfections were accomplished by electroporation (Neon) using the manufacturer’s instructions.

Vorselen D., Barger S.R., Wang Y., Cai W., Theriot J.A., Gauthier N.C, & Krendel M. (2021). Phagocytic ‘teeth’ and myosin-II ‘jaw’ power target constriction during phagocytosis. eLife, 10, e68627.

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Fluorescence Imaging of Podosome Proteins

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Human myo1e and myo1f constructs tagged with EGFP, mEmerald-C1, or mScarlet-C1 have been previously described 12 . mEmerald-Lifeact was a gift from Michael Davidson (Addgene #54148). Chicken regulatory light chain (RLC) tagged with EGFP was a gift from Klaus Hahn. WASP tagged with myc was a gift from Dianne Cox, and was subcloned into pUB-Halo-C1 vector.
CMV-GFP-NMHCII-A was a gift from Robert Adelstein (Addgene #11347). ARP3-mCherry (Addgene #27682) and mCherry-cortactin (Addgene #27676) were gifts from Christien Merrifield that were subcloned into EGFP-C1 and mEmerald-C1, respectively. Chicken paxillin was a gift from Chris Turner, which was subcloned into mScarlet-i-C1. Chicken vinculin was a gift from Kenneth Yamada (Addgene #50513) and subcloned into pUB-mEmerald-C1.
Immunohistochemical staining to determine localization of select podosome-related proteins in relation to the actin teeth did not produce good results, which may be due to the adhesive and porous nature of the IgG-functionalized DAAMPs. As an alternative, we transfected the RAW macrophages with fluorescently tagged proteins. All transfections were accomplished by electroporation (Neon) using the manufacturer's instructions.

Vorselen D., Barger S.R., Wang Y., Cai W., Theriot J.A., Gauthier N.C., & Krendel M. (2021). Phagocytic “teeth” and myosin-II “jaw” power target constriction during phagocytosis.

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