Anti α4β7

Manufactured by BioLegend

Sourced in United States

Anti-α4β7 is a lab equipment product that targets the α4β7 integrin, a protein involved in cell adhesion and migration. This product can be used for research purposes to detect and study the α4β7 integrin in biological samples.

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3 protocols using anti α4β7

Visualizing Intestinal Immune Cells

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Small intestine tissues were stained with anti-CD4 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-IL-17 (Abcam), and anti-α4β7 (Biolegend, San Diego, CA, USA) primary antibodies at 4°C overnight, followed by secondary antibodies conjugated with FITC (Santa Cruz Biotechnology, APC (Thermo Fisher Scientific, Rockford, IL, USA) and PE (Thermo Fisher Scientific) incubated at room temperature for 2 h. Nuclei were stained with 4,’6-diamidino-2-phenylindole (DAPI; Invitrogen, Carlsbad, CA). Confocal images were analyzed using an LSM 700 confocal microscope (Zeiss, Oberkochen, Germany) at 200× magnification.

Hwang S.H., Woo J.S., Moon J., Yang S., Park J.S., Lee J., Choi J., Lee K.H., Kwok S.K., Park S.H, & Cho M.L. (2021). IL-17 and CCR9+α4β7– Th17 Cells Promote Salivary Gland Inflammation, Dysfunction, and Cell Death in Sjögren’s Syndrome. Frontiers in Immunology, 12, 721453.

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Multiparametric Flow Cytometry Immune Profiling

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Cell suspensions were stained with: anti-CD45 (30-F11); anti-CD45.1 (A20); anti-CD45.2 (104); anti-CD11c (N418); anti-CD11b (Mi/70); anti-CD127 (IL7Rα; A7R34); anti-CD27(LG.7F9); anti-CD8α (53-6.7); anti-CD19 (eBio1D3); anti-CXCR4(L276F12); anti-NK1.1 (PK136); anti-CD3ε (eBio500A2); anti-TER119 (TER-119); anti-Gr1 (RB6-8C5); anti-CD4 (RM4-5); anti-CD25 (PC61); anti-CD117 (c-Kit; 2B8); anti-CD90.2 (Thy1.2; 53-2.1); anti-TCRβ (H57-595); anti-TCRγδ (GL3); anti-B220 (RA3-6B2); anti-KLRG1 (2F1/KLRG1); anti-Ly-6A/E (Sca1; D7); anti-CCR9 (CW-1.2); anti-IL-17 (TC11-18H10.1); anti-rat IgG1k isotype control (RTK2071); anti-streptavidin fluorochrome conjugates from Biolegend; anti-α4β7 (DATK32); anti-Flt3 (A2F10); anti-NKp46 (29A1.4); anti-CD49b (DX5); anti-Ki67 (SolA15); anti-rat IgG2ak isotype control (eBR2a); anti-IL-22 (1H8PWSR); anti-rat IgG1k isotype control (eBRG1); anti-EOMES (Dan11mag); anti-Tbet (eBio4B10); anti-FOPX3 (FJK-16s); anti-GATA3 (TWAJ); anti-CD16/CD32 (93); 7AAD viability dye from eBiosciences; anti-CD196 (CCR6; 140706) from BD Biosciences; anti-RORγt (Q31-378) and anti-mouse IgG2ak isotype control (G155-178) from BD Pharmingen. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit was purchased from Invitrogen.

Godinho-Silva C., Domingues R.G., Rendas M., Raposo B., Ribeiro H., Alves da Silva J., Vieira A., Costa R.M., Morais-Barbosa N.L., Carvalho T, & Veiga-Fernandes H. (2019). Light-entrained and brain-tuned circadian circuits regulate ILC3 and gut homeostasis. Nature, 574(7777), 254-258.

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Multiparametric Flow Cytometry Immune Profiling

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