Accupower cyclescript rt premix cdna synthesis kit

Total RNA was extracted using an easy-spin Total RNA Extraction Kit (Intron Biotechnology, Seongnam, Republic of Korea), according to the manufacturer’s instructions. RNA samples were then quantified, aliquoted, and stored at − 80 °C until analysis. Total RNA (500 ng) was reverse-transcribed to cDNA using an AccuPower CycleScript RT PreMix cDNA synthesis kit (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions. qPCR was performed in a total volume of 20 μL containing 7.2 μL of PCR-grade distilled water, 0.4 μL of forward and reverse primers, and 10 μL of the SYBR Green I Master mix (Roche Diagnostics, Mannheim, Germany). The PCR conditions were as follows: 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s, 59 °C for 15 s, and 72 °C for 15 s. All primers were synthesized by Bioneer Corp. (Daejeon, Republic of Korea). Relative mRNA expression levels were normalized to ACTB mRNA levels.

Total RNA was extracted using an easy-spin™ Total RNA Extraction Kit (Intron Biotechnology, Seongnam, Republic of Korea) according to the manufacturer’s instructions. RNA samples were then quantified, aliquoted, and stored at − 80 °C until analysis. Total RNA (500 ng) was reverse transcribed into cDNA using an AccuPower CycleScript RT PreMix cDNA synthesis kit (Bioneer, Daejeon, Republic of Korea) according to the manufacturer’s instructions. RT-qPCR was conducted in a total volume of 20 μL containing 7.2 μL of PCR-grade distilled water, 0.4 μL of forward and reverse primers, and 10 μL of the SYBR Green I Master mix (Roche Diagnostics, Mannheim, Germany). PCR conditions were as follows: 95 °C for 10 min, followed by 35 cycles of 95 °C for 10 s, 55 °C for 10 s, and 72 °C for 10 s. All primers were synthesised by Bioneer Corp. (Daejeon, Republic of South Korea). The relative mRNA expression levels were normalised to β-actin mRNA levels.