4700 proteomics analyser
The 4700 Proteomics Analyzer is a mass spectrometry instrument designed for the analysis of proteins. It features a time-of-flight (TOF) analyzer and a matrix-assisted laser desorption/ionization (MALDI) ion source. The instrument is capable of performing high-throughput analysis of protein samples.
6 protocols using 4700 proteomics analyser
MALDI-ToF-MS Analysis of Enriched Fructans
MALDI-ToF/ToF-MS/MS Analysis of Glycans
Per-methylated samples were analysed by MALDI-ToF/ToF-MS/MS (4700 Proteomics Analyser, Applied Biosystems, Foster City, CA, USA) as previously described [50] (link), using 2,5-dihydroxybenzoic acid (2,5-DHB) matrix (10 mg ml−1 dissolved in 50% MeOH). The above tandem mass spectrometer uses a 200 Hz frequency triple Nd-YAG laser operating at 355 nm wavelength. High energy MALDI-CID spectra were acquired with an average 10,000 laser shots/spectrum, using a high collision energy (1 kV). The oligosaccharide ions were allowed to collide in the CID cell with argon at a pressure of 2×10−6 Torr.
In-Gel Tryptic Digestion and MALDI-TOF-TOF MS
Subsequently, the peptide mixtures were re-dissolved in 0.5% TFA, and 1 µl of peptide solution was mixed with an equal volume of matrix (4-hydroxy-a-cyanocinnamic acid in 30% ACN/ 0.1% TFA). Then, the peptides were spotted on the target plate. Individual protein peptides were identified by MALDI-TOF-TOF mass spectrometry on a 4700 Proteomics Analyser (Applied Biosystems, Foster City, California, USA). The mass spectra were used to examine human protein sequences in the Swiss-Prot database using the Mascot database search algorithm (version 1.9).
MALDI-ToF-MS/MS Analysis of Oligosaccharides
Protein Identification via MALDI-TOF MS
MALDI-TOF-MS Analysis of Glycans
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