Aba test kit

Manufactured by Agdia

Sourced in United States

The ABA Test Kit is a laboratory equipment product designed to detect the presence of abscisic acid (ABA) in plant samples. It provides a quantitative analysis of ABA levels, enabling researchers and scientists to study the physiological responses of plants to various environmental conditions.

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3 protocols using aba test kit

Measuring ABA Content in Cold-Stressed Plants

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ABA content was measured as previously described [112 (link)]. For cold stress, we transferred the plantlets (4-week-old) to 0 °C (freezing chamber RuMED4001) for 0 h, 2 h, 4 h, 6 h under dim light (0.7-0.8 μmol sec-1 m-2). After cold stress, a total of 20 mg of homogenized M. falcata (PI 502449) and M. truncatula (A17) leaves were soaked in 1 ml of ABA extraction buffer (10 mM HCl and 1 % polyvinylpolypyrrolidone in methanol) and shaked at 4 °C for 16 h. After neutralization with 15 μl of 1 M NaOH, the supernatant was dried and resuspended in TBS. The ABA content was quantified using a Phytodetek ABA Test Kit (Agdia, USA). These experiments were repeated three times with similar results.

Miao Z., Xu W., Li D., Hu X., Liu J., Zhang R., Tong Z., Dong J., Su Z., Zhang L., Sun M., Li W., Du Z., Hu S, & Wang T. (2015). De novo transcriptome analysis of Medicago falcata reveals novel insights about the mechanisms underlying abiotic stress-responsive pathway. BMC Genomics, 16, 818.

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ABA Quantification Using Phytodetek Kit

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ABA content was measured with a Phytodetek ABA test kit (Agdia, Inc., Elkhart, IN) following the manufacturer’s instructions.

Yan J., Zhao C., Zhou J., Yang Y., Wang P., Zhu X., Tang G., Bressan R.A, & Zhu J.K. (2016). The miR165/166 Mediated Regulatory Module Plays Critical Roles in ABA Homeostasis and Response in Arabidopsis thaliana. PLoS Genetics, 12(11), e1006416.

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ABA Quantification in Salt-Stressed Arabidopsis

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ABA concentration (nmol·L⁻¹) was quantified by indirect enzyme-linked assay (ELISA) using a Phytodetek ABA test Kit (Agdia, Elkhart, IN, USA) on 3 plants per treatment from T9 (Mix), T1 (MOT1^DEL) and PA10 (MOT1^C) demes along with Col-0 and mot1-2, copt6-1 and aba2-1 mutants cultivated in common soil irrigated with 0 and 50 mM of NaCl for 2 weeks.

Busoms S., Terés J., Yant L., Poschenrieder C, & Salt D.E. (2021). Adaptation to coastal soils through pleiotropic boosting of ion and stress hormone levels in wild Arabidopsis thaliana. The New phytologist, 232(1), 208-220.

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