STEM and HRTEM images were recorded on a Cs-corrected Titan 80–300 microscope operated at 300 kV. X-ray diffraction patterns were obtained using an X-ray diffractometer (Bruker, Germany) with Cu-Kα radiation. The 2θ scanning range was 10° to 80° with a scanning speed of 0.1° s−1. XPS was performed using an ESCALAB 250 spectrometer with Al Kα X-ray excitation (1,486.6 eV). Raman spectra were measured using an Invia-Reflex Raman system using a 785-nm laser. UV–vis and fluorescence spectra were obtained using Hitachi U-3010 and F-4500 spectrophotometers, respectively. ROS were detected using the ESR technique (ESP300E, Bruker).
Esp 300e
Manufactured by Bruker
Sourced in United States
The ESP 300E is a compact and versatile electron spin resonance (ESR) spectrometer designed for a wide range of applications. It features a stable and sensitive detection system, allowing for the analysis of various paramagnetic samples. The ESP 300E provides accurate measurements and reliable data for researchers and scientists in various fields.
Automatically generated - may contain errors
Lab products found in correlation
F 4500 spectrophotometer, by Hitachi (1 mentions)
U 3010, by Hitachi (1 mentions)
X ray diffractometer, by Bruker (1 mentions)
3t triotim, by Siemens (1 mentions)
Escalab 250xi, by Thermo Fisher Scientific (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Cary 5000, by Agilent Technologies (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Esr900, by Oxford Instruments (1 mentions)
Avance 250, by Bruker (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Benzyl alcohol, by Merck Group (1 mentions)
Glovebox, by MBraun (1 mentions)
4 tert butylphenol, by Merck Group (1 mentions)
9 protocols using esp 300e
1
Characterization of Advanced Materials
2
Comprehensive Nanomaterial Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transmission electron microscopy (TEM) and high-resolution TEM (HRTEM) images were acquired using a JEM-2100F (JEOL, Tokyo, Japan). Atomic force microscopy (AFM) was performed with a Veeco (New York, NY, USA) microscope. X-ray photoelectron spectroscopy (XPS) was conducted using a Thermo Fisher Scientific Escalab 250Xi. X-ray diffraction (XRD) patterns were obtained using a Max-3A (Rigaku, Tokyo, Japan). Ultraviolet (UV)-visible spectroscopy measurements were acquired with a Cary 5000 (Agilent Technologies, Santa Clara, CA, USA). ζ-Potential measurements and dynamic light-scattering (DLS) analysis were performed using Zetasizer Nano ZS90 (Malvern Instruments, Malvern, UK). ROS were observed using electron-spin resonance (ESR; ESP300E; Bruker, Billerica, MA, USA). To monitor temperature changes at tumor sites during irradiation, IR thermal images were observed using a PTT monitoring system (MG33; Magnity Electronics, Shanghai, China). Fluorescence and photoluminescence (PL) spectra were collected using fluorescence spectrophotometry (FluoroSens; Gilden Photonics, Glasgow, UK). Absorbance for MTT assays was assessed with a Thermo Reader at a wavelength of 490 nm. MRI (Trio TIM 3 T; Siemens, Munich, Germany) was employed to test T2 relaxation times. Details of the methods used for material characterization are described in the Supplementary material .
3
Probing Complex I via EPR Spectroscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Low temperature cw-EPR spectra were obtained using a Bruker ESP 300E spectrometer equipped with a liquid helium continuous flow cryostat, ESR 900 (Oxford Instruments). Samples of purified complex I were diluted to 5 mg protein ml−1 in 20 mM Hepes pH 7.5, 100 mM NaCl, incubated with either 5 mM DTT or 0.1 mM DTNB for 5 min on ice and then mixed with 2 mM NADH in the EPR tube and frozen in liquid nitrogen after 30 s reaction time. Spectra were recorded as described earlier26 (link) using instrument settings as listed in the figure legend.
4
EPR Spin Trapping for Superoxide Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All EPR measurements
were performed
using Bruker ER 300 or ESP 300E spectrometers operating at X-band
with TM110 cavity resonators. The microwave frequency was
measured with an EIP model 575 microwave counter (EIP Microwave, Inc.,
San Jose, CA). To assess• O2– generation, EPR spin trapping studies
were performed using the spin trap DEPMPO.22 (link),23 (link) The following instrument settings were used in the spin trapping
experiments: modulation amplitude, 0.32 G; time constant, 0.16 s;
scan time, 60 s; modulation frequency, 100 kHz; microwave power, 20
mW; microwave frequency, 9.76 GHz. The samples were placed in a quartz
EPR flat cell, and spectra were recorded at ambient temperature (25
°C). The collection of EPR data was started 2 min after the addition
of xanthine. The component signals observed in these spectra were
identified and quantified as reported. The double integrals of DEPMPO-OOH
experimental spectra were compared with those of a 1 μM TEMPO
sample measured under identical settings to estimate the concentration
of the• O2– adduct.22 (link),23 (link)
were performed
using Bruker ER 300 or ESP 300E spectrometers operating at X-band
with TM110 cavity resonators. The microwave frequency was
measured with an EIP model 575 microwave counter (EIP Microwave, Inc.,
San Jose, CA). To assess
were performed using the spin trap DEPMPO.22 (link),23 (link) The following instrument settings were used in the spin trapping
experiments: modulation amplitude, 0.32 G; time constant, 0.16 s;
scan time, 60 s; modulation frequency, 100 kHz; microwave power, 20
mW; microwave frequency, 9.76 GHz. The samples were placed in a quartz
EPR flat cell, and spectra were recorded at ambient temperature (25
°C). The collection of EPR data was started 2 min after the addition
of xanthine. The component signals observed in these spectra were
identified and quantified as reported. The double integrals of DEPMPO-OOH
experimental spectra were compared with those of a 1 μM TEMPO
sample measured under identical settings to estimate the concentration
of the
5
ESR Spin Trapping for Photogenerated Free Radicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ESR spin trapping was used to monitor photogeneration of short-lived free radicals during irradiation of samples with visible light in a flat ESR cell (Wilmad Glass Co., Vineland, NJ, USA) with an optical pathlength of 0.3 mm in situ in the resonant cavity, as described previously [6 (link),13 (link),14 (link),15 (link)]. Samples contained suspensions of oxidised 16:0;22:6PC liposomes in phosphate-buffered saline (PBS) or liposomal extracts of oxidised DHA solubilised in DMSO in the presence of 100 mM DMPO used as a spin trap. ESR spectra were recorded using the ESR spectrometer (ESP 300E; Bruker, Billerica, MA, USA) operating at 9.5 GHz with 100-kHz field modulation. To distinguish between primary and secondary products, the time course of formation of DMPO adducts and their decay was followed both in the dark and during irradiation with light.
6
Validation of ROS Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Quenching experiments were performed to validate the production of ROSs. TBA and p-BQ were used to quench hydroxyl radicals (HO • ) and superoxide anion radicals ( ), respectively. One scavenger was added to the solution at the start of the quenching tests, but all other protocols were kept the same. The active radicals were identi ed using electron paramagnetic resonance (EPR, Bruker ESP-300E, Germany) spectroscopy, and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) was used to spin trap HO • and radicals in order to form corresponding adducts. The EPR measurements were performed using 10 mW of microwave power, 100 G of scan range, and 1 G of eld modulation.
7
Comprehensive Analytical Techniques for Chemical Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
General Methods 1 H NMR spectra were recorded using Bruker Avance 250, 400, 500 instruments and Me4Si as an internal standard. Infrared spectra were recorded with a Spectrum One FT-IR Spectroscopy instrument and UV/Vis/NIR spectra were measured using a Cary 5000E Varian. ESR spectra were performed with a Bruker ESP 300 E equipped with a rectangular cavity T102 that works with an X-band (9.5 GHz). The solutions were degassed by argon bubbling before the measurements. LDI/TOF MS were recorded in a Bruker Ultraflex LDI-TOF spectrometer. Cyclic voltammetry measurements were obtained with a potentiostat 263a from EG&G Princeton Applied Research in a standard 3 electrodes cell. All reagents and solvents employed for the syntheses were of high purity grade and were purchased from Sigma-Aldrich Co., Merck, and SDS. Dry solvents were used in the chemical reactions and in the cyclic voltammetries. The solvents used for optical spectroscopy and ESR measurements were of HPLC grade (ROMIL-SpS). In addition, for cyclic voltammetry experiments, CH2Cl2 was filtered over basic alumina to eliminate the acidic residues.
8
Spectrophotometric and EPR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Spectrophotometric experiments were carried out with a UV-VIS spectrophotometer Model Ultrospec 2110pro using the full range of wavelengths between 200 and 900 nm.
EPR experiments were performed at room temperature with a Bruker spectrometer (ESP300e) equipped with a TMH cavity. The amplitude of the modulation signals was varied between 0.001 and 0.02 mT depending on the sample. The microwave power was set to 0.2 or 0.63 mW. Spectra were recorded with variable scan times up to 250 s and were consecutively stored in order to monitor the kinetic behavior of the radicals. The samples were prepared either at pH = 12 or pH = 13 in the presence or absence of oxygen (purging solutions with nitrogen), and were filled into a flat cell (Wilmad) under nitrogen atmosphere.
EPR experiments were performed at room temperature with a Bruker spectrometer (ESP300e) equipped with a TMH cavity. The amplitude of the modulation signals was varied between 0.001 and 0.02 mT depending on the sample. The microwave power was set to 0.2 or 0.63 mW. Spectra were recorded with variable scan times up to 250 s and were consecutively stored in order to monitor the kinetic behavior of the radicals. The samples were prepared either at pH = 12 or pH = 13 in the presence or absence of oxygen (purging solutions with nitrogen), and were filled into a flat cell (Wilmad) under nitrogen atmosphere.
9
Organometallic Synthesis and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reactions and operations were performed under an inert atmosphere of N 2 while using a glove-box (MBraun) or standard Schlenk apparatus and vacuum line techniques. The sol-vents were purified by standard methods: methanol, distilled from Mg; toluene, distilled from Na; CH 2 Cl 2 , distilled from P 2 O 5 ; hexanes, distilled from Na; C 6 D 6 , distilled from CaH 2 . L-LA ((3S)-cis-3,6-dimethyl-1,4-dioxane-2,5-dione) (98%; Aldrich) was sublimed and recrystallized from toluene prior to use. Benzyl alcohol (Aldrich) was distilled under dry nitrogen gas and freeze/thaw degassed prior to use. ZnEt 2 (1.0 M solution in hexanes), 4-tert-butylphenol, 2-methylaminomethyl-1,3dioxolane (98%), and formaldehyde (37% solution in H 2 O) were purchased from Aldrich and used as received. (L R ZnEt) 2 was obtained according to the literature method. 44 1 H and 13 C NMR spectra were detected over a temperature range from 233 to 333 K using Bruker ESP 300E or 500 MHz spectrometers. Chemical shifts are reported in parts per million and referenced to residual protons in deuterated solvents. Microanalyses were conducted with an ARL Model 3410 + ICP spectrometer (Fisons Instruments) and a VarioEL III CHNS (in-house).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!