Imh 360

Human HCC tissue microarrays were purchased from Imgenex Corp. Two tissue microarrays were used: one containing 40 primary HCC, 10 metastatic HCC, and 9 normal adjacent liver samples (Imgenex; IMH‐360), the other containing 46 primary HCC and 13 metastatic HCC (Imgenex; IMH‐318). Antigen retrieval was performed by the Department of Pathology, VCU (with thanks to Dr. George Alemara). Immuno‐staining was performed using anti‐PDE5 antibody (1:50) or using a mixture of anti‐PDGFRα and anti‐PDGFRβ antibodies (1:50, each). After immuno‐staining, slides were H&E stained and slides examined using a confocal microscope (×10).

Human HCC tissue microarray, containing 40 primary HCC, 10 metastatic HCC and 9 normal adjacent liver samples was obtained from Imgenex Corp (San Diego, CA, Catalogue# IMH-360) [22] (link). Out of the 50 HCC samples, 14 belongs to Stage I, 9 belongs to Stage II, 14 belongs to Stage III and 13 belongs to Stage IV. Following deparaffinization, the sections were permeabilized with a 0.1% TritonX-100 solution in PBS for 30 min. Sections were then blocked for 1 h at room temperature with 2% goat serum and 1% BSA in PBS and incubated with anti-SND1 antibody (rabbit polyclonal; 1:100; Prestige Antibodies® Powered by Atlas Antibodies from Sigma) or anti-AT1R antibody (1:200; rabbit polyclonal; Abnova) overnight at 4 °C. Sections were then rinsed in PBS, and incubated with anti-rabbit secondary antibody for 1 h at room temperature. The signals were developed by avidin–biotin–peroxidase complexes with a DAB substrate solution (Vector laboratories). The images were taken by an Olympus microscope.