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Trigalacturonic acid

Manufactured by Merck Group
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Sourced in United States, Germany

Trigalacturonic acid is a chemical compound that is used as a laboratory reagent. It is a component of pectin, a polysaccharide found in plant cell walls. Trigalacturonic acid is often used in research and analysis applications that involve the study of plant-derived compounds and materials.

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Lab products found in correlation

Citrus pectin, by Merck Group (2 mentions) Apple pectin, by Merck Group (2 mentions) Digalacturonic acid, by Merck Group (2 mentions) Polygalacturonic acid, by Merck Group (2 mentions) Polygalacturonic acid sodium salt, by Merck Group (1 mentions) D galacturonic acid monohydrate, by Merck Group (1 mentions) Dna restriction endonuclease, by Takara Bio (1 mentions) D galacturonic acid, by Merck Group (1 mentions) La taq dna polymerase, by Takara Bio (1 mentions) T4 dna ligase, by Takara Bio (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Salicylic acid, by Merck Group (1 mentions) Tyrosol, by Merck Group (1 mentions) Xylose, by Merck Group (1 mentions) Arabinose, by Merck Group (1 mentions) 4 hydroxybenzoic acid, by Merck Group (1 mentions) Galactose, by ITW Reagents (1 mentions) Mannose, by ITW Reagents (1 mentions) Fructose, by ITW Reagents (1 mentions) Galacturonic acid monohydrate, by Merck Group (1 mentions)

5 protocols using trigalacturonic acid

1

Characterization of Oligogalacturonides

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Commercially available trimers (Trigalacturonic acid, Sigma-Aldrich) and a long-OG mix with a stated DP10-15 (Elicityl) were run on Silica gel 60F254 TLC plates and analyzed by mass spectrometry to confirm the degree of polymerization. Trimers were found to consist of only DP3, whereas the long-OG mix was found to consist of primarily DP8-19, with an average DP of approximately 14 (Additional file 10: Figure S3, Additional file 11: Figure S4 and Additional file 12: Figure S5).
Davidsson P., Broberg M., Kariola T., Sipari N., Pirhonen M, & Palva E.T. (2017). Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana. BMC Plant Biology, 17, 19.
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2

Pectin Purification and Characterization

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Polygalacturonic acid sodium salt, D-galacturonic acid monohydrate, digalacturonic acid, trigalacturonic acid, citrus pectin (esterification degree of 34% and 90%), apple pectin (esterification degree of 75%), and chromatographic resins were all purchased from Sigma. citrus pectin (esterification of 72%) was kindly donated by CP Kelco (Limeira, SP, Brazil).
Pedrolli D.B, & Carmona E.C. (2014). Purification and Characterization of a Unique Pectin Lyase from Aspergillus giganteus Able to Release Unsaturated Monogalacturonate during Pectin Degradation. Enzyme Research, 2014, 353915.
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3

Pectins and Enzymatic Modifications

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LA Taq DNA polymerase, DNA restriction endonuclease, T4 DNA ligase and RNA purification reagent RNAiso Plus were obtained from TaKaRa Bio Co. Ltd. (China). D-galacturonic acid, trigalacturonic acid, polygalacturonic acid (PGA, de-esterified), citrus pectin (<26% esterified) and apple pectin (50–75% esterified) were from Sigma-Aldrich Co. Ltd. (USA). Unless otherwise stated, all other chemicals were of analytical grade.
Lu B., Xian L., Zhu J., Wei Y., Yang C, & Cheng Z. (2022). A Novel Endo-Polygalacturonase from Penicillium oxalicum: Gene Cloning, Heterologous Expression and Its Use in Acidic Fruit Juice Extraction. Journal of Microbiology and Biotechnology, 32(4), 464-472.
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4

Characterization of Polysaccharides and Phenolics

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The standards used for determination were: arabinose (99%), xylose (99%), rhamnose monohydrate (99%), galacturonic acid monohydrate (97%), acetic acid (96%), formic acid (99%), gallic acid (97.5%), trolox (97%), digalacturonic acid (85%), trigalacturonic acid (90%) and polygalacturonic acid (90%), purchased from Sigma-Aldrich (Steinheim, Germany); glucose (98%), purchased from Scharlau (Barcelona, Spain); galactose (99%), mannose (99%) and fructose (98%), purchased from Panreac (Barcelona, Spain). For the identification of phenolic compounds, 4-hydroxybenzoic acid (99%), p-coumaric acid (98%), gallic acid (97.5%), salicylic acid (99%), tyrosol (98%), sinapic acid (98%) and caffeic acid (98%) were purchased from Sigma-Aldrich (Steinheim, Germany); ferulic acid (99%) and vanillic acid (97%) were purchased from Sigma-Aldrich-Fluka (Steinheim, Germany).
Rico X., Gullón B, & Yáñez R. (2020). Environmentally Friendly Hydrothermal Processing of Melon by-Products for the Recovery of Bioactive Pectic-Oligosaccharides. Foods, 9(11), 1702.
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5

Alginate Oligomers and Trimannuronate Synthesis

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Guluronic acid oligomers (G oligomer) with two different molecular weight distributions were prepared 10 from partially acid hydrolysed, high guluronate alginate from Laminaria hyperborea stipes by acid precipitation to give oligomers with a number average degree of polymerization (DPn) of 21 and a fraction of guluronic acid (FG) of 0.90 (determined by NMR). Samples with DPn of 4 and 10 were prepared by further hydrolysis.
Trimannuronate was an inhouse sample prepared according to previously described methods. 11, 12 Dextran T-2000 (Mw = 2 000 000 g/mol) was purchased from Pharmacia Fine Chemicals. Maltotriose, trigalacturonic acid, adipic acid dihydrazide (ADH), O,O'-1,3,-propanediylbishydroxylamine dihydrochloride (PDHA) and 2-methylpyridine borane complex (α-picoline borane) was purchased from Sigma-Aldrich. PEG linkers were purchased from BroadPharma, US. All other chemicals were obtained from commercial sources and of analytical grade.
Solberg A., Mo I.V., Aachmann F.L., Schatz C., & Christensen B.E. (2021). Alginate-based diblock polymers: preparation, characterization and Ca-induced self-assembly. Polymer Chemistry, 12(38), 5412-5425.
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