Purified rat anti mouse ifn γ

DNCB (2, 4-dinitrochlorobenzene), mite extract, phorbol 12-myristate 13-acetate (PMA), A23187, and carboxyfluoresceinsuccinimidyl ester (CFSE) were purchased from Sigma (St. Louis, MO). FITC-anti-mouse CD4, PerCP cy5.5-anti-mouse IFN-γ, PE-anti-mouse IL-4, and FITC-anti-mouse CD4 were obtained from e-Bioscience (San Diego, CA). A mouse IgE ELISA kit, purified rat anti-mouse IFN-γ, and purified rat anti-mouse IL-12 were obtained from BD Biosciences (San Jose, CA). Mouse anti-CD28, mouse IL-4 ELISA kit, recombinant human IFN-γ, and recombinant human TNF-α were purchased from R&D Systems (Minneapolis, MN). Recombinant mouse IL-4 was obtained from Peprotech (Hamburg, Germany). The 145-2C11 (mouse anti-CD3; CRL-1975) hybridoma cell line was purchased from the ATCC (Manassas, VA). HaCaT keratinocytes were cultured in RPMI 1640 containing 2 mM L-glutamine, antibiotics (100 μg/mL streptomycin, 100 U/mL penicillin), and 10% fetal bovine serum. Cells were incubated at 37°C in a humidified atmosphere of 5% CO₂.

All commercial antibodies and reagents were purchased from the following resources: anti-phospho-cPLA2 (Ser505) substrate (#2831, 1: 1000 dilution), anti-cPLA2 (#2832, 1: 1000 dilution), anti-phospho-HSP27 (Ser82) (#2401, 1: 1000 dilution), anti-HSP27 (#2402, 1: 1000 dilution), and anti-ApoA1 (#3350, 1: 1000 dilution) antibodies were from Cell Signaling Technology. Ovalbumin (A5503) was purchased from Sigma-Aldrich (St. Louis, USA). Purified rat anti-mouse IgE (R35-72), purified rat anti-mouse IgG1 (A85-3), purified rat anti-mouse IgG2a (R11-89), purified rat anti-mouse IL-4, purified rat anti-mouse IL-5, purified rat anti-mouse IFN-γ, biotin rat anti-mouse IgE (R35-118), biotin rat anti-mouse IgG1 (A85-1), biotin rat anti-mouse IgG2a (19-5), biotin rat anti-mouse IL-4, biotin rat anti-mouse IL-5, and biotin rat anti-mouse IFN-γ were purchased from BD Biosciences (San Diego, USA).

Ovalbumin (A5503) and astaxanthin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Purified rat anti-mouse IL-4, purified rat anti-mouse IL-5, purified rat anti-mouse IFN-γ, biotin rat anti-mouse IL-4, biotin rat anti-mouse IL-5, biotin rat anti-mouse IFN-γ purified rat anti-mouse IgE (R35-72), purified rat anti-mouse IgG1 (A85-3), purified rat anti-mouse IgG2a (R11-89), biotin rat anti-mouse IgE (R35-118), biotin rat anti-mouse IgG1 (A85-1), and biotin rat anti-mouse IgG2a (19-5) were purchased from BD Biosciences (San Diego, CA, USA).