Immunoblotting was the same as previously described [26 (link)]. The following primary antibodies were used: TXNIP (Novus Biologicals), phospho-ERK1/2 (p-ERK; Cell Signaling, Denver, MA, USA), total ERK (t-ERK; Cell Signaling), phospho-JNK (p-JNK; Cell Signaling), total JNK (t-JNK; Cell Signaling), phospho-p38 (p-p38; Cell Signaling), total p38 (t-p38; Cell Signaling), AP-1 (Abcam), and β-actin (Cell Signaling). The relative protein expression was determined using ChemiDoc (Bio-Rad Laboratories).
Total jnk t jnk
Manufactured by Cell Signaling Technology
Sourced in United States
Total JNK (t-JNK) is an antibody that detects the total amount of JNK (c-Jun N-terminal kinase) protein in cells or tissues. JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in various cellular processes, including stress response, cell growth, and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Chemidoc, by Bio-Rad (1 mentions)
Phospho p38 p p38, by Cell Signaling Technology (1 mentions)
Phospho jnk p jnk, by Cell Signaling Technology (1 mentions)
Phospho erk1 2 p erk, by Cell Signaling Technology (1 mentions)
Total erk t erk, by Cell Signaling Technology (1 mentions)
Total p38 t p38, by Cell Signaling Technology (1 mentions)
Txnip, by Novus Biologicals (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Merck Group (1 mentions)
Poly adp ribose polymerase 1 parp1, by Cell Signaling Technology (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
Phosphorylated jnk, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
β catenin, by Cell Signaling Technology (1 mentions)
Primary antibodies against α tubulin, by Merck Group (1 mentions)
2 protocols using total jnk t jnk
1
Immunoblotting Analysis of Signaling Pathways
2
Protein Expression Analysis by Western Blot
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Western blot analysis was performed as previously described [16 ]. Primary antibodies against Bax (rabbit, diluted 1:1000), poly(ADP-ribose) polymerase 1 (PARP1) (rabbit, diluted 1:1000), caspase-3 (rabbit, diluted 1:1000), β-catenin (rabbit, diluted 1:1000), phosphorylated (P)-JNK (rabbit, diluted 1:1000), and total-JNK (T-JNK) (rabbit, diluted 1:1000) were purchased from Cell Signaling Technology. Antibodies against sFRP1 (rabbit, diluted 1:500) were obtained from Cloud-Clone Corp. (CCC, USA). Primary antibodies against α-tubulin (diluted 1:5000, Sigma, USA) and glyceraldehyde 3-phosphate dehydrogenase (diluted 1:1000, Sigma, USA) served as loading controls for whole-cell lysates. The band intensity was analyzed by Quantity One software (Bio-Rad, USA).
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