Xuebijing injection (catalog number: z20040033, batch number: 1905061) was manufactured by Tianjin Chase Sun Pharmaceutical Co., Ltd (Tianjin, China). Paeoniflorin (CAS #:23180-57-6), Hydroxysafflor yellow A (CAS #: 78281-02-4), Ferulic acid (CAS # 537- 98-4), and protocatechuic aldehyde (CAS #: 139-85-5) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Mouse NT-proBNP ELISA (Cat#: ZC-37812-96T) kit, DCFH-DA (Cat#:CA1410-100T), RIPA tissue/cell lysate (Cat#: R0020), SDS-PAGE gel kit, and Rainbow 245 Spectrum Marker (Cat#:PR 1920), Mouse IL-6 ELISA kit (Cat#:ZC-37988-96T), Mouse IL-1β ELISA kit (Cat#:ZC-37974-96T), and EasySee Western Blot Kit (Lot#:O30807) were purchased from Solarbio (Beijing, China). The primary antibody of rabbit anti-CXCL2 (bs-1162R) was purchased from Bioss Inc., (Beijing, China) and GAPDH, 1:4,000 (14C10) was from Cell Signaling Technology (Beverly, MA, United States). The secondary antibody Goat anti-Rabbit IgG (1:4,000) (ZB-2301) was purchased from ZS bio (Beijing, China). 2, 2, 2-Tribromoalcohol (Cat#:T48402) and Bacterial lipopolysaccharide (Cat#: L2880-10 MG) were purchased from Sigma Aldrich (St. Louis, MO, United States). Rhod2-AM (Cat#: zy0129) and Anti-GRP78 (BiP) antibody (ab21685) were purchased from Abcam (Cambridge, MA, United States).
Protocatechuic aldehyde
Manufactured by Yuanye Bio-Technology
Sourced in China, United States
Protocatechuic aldehyde is a chemical compound used in laboratory settings. It serves as a substrate for various enzymatic reactions and is commonly utilized in analytical assays and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Cell Signaling Technology (1 mentions)
Paeoniflorin, by Yuanye Bio-Technology (1 mentions)
Dcfh da, by Solarbio (1 mentions)
Ripa tissue cell lysate, by Solarbio (1 mentions)
Mouse il 6 elisa kit, by Solarbio (1 mentions)
Mouse il 1β elisa kit, by Solarbio (1 mentions)
Bacterial lipopolysaccharide, by Merck Group (1 mentions)
Hydroxysafflor yellow a, by Yuanye Bio-Technology (1 mentions)
Ferulic acid, by Yuanye Bio-Technology (1 mentions)
Rhod 2 am, by Abcam (1 mentions)
Sds page gel kit, by Solarbio (1 mentions)
Dextran sulfate sodium (dss), by MP Biomedicals (1 mentions)
Radio immunoprecipitation assay ripa buffer, by Beyotime (1 mentions)
Tunel apoptosis assay kit, by Beyotime (1 mentions)
L arginine, by Yuanye Bio-Technology (1 mentions)
L tryptophan, by Yuanye Bio-Technology (1 mentions)
Caffeic acid, by Yuanye Bio-Technology (1 mentions)
Gallic acid, by Yuanye Bio-Technology (1 mentions)
Amygdalin, by Yuanye Bio-Technology (1 mentions)
Phenylalanine, by Yuanye Bio-Technology (1 mentions)
4 protocols using protocatechuic aldehyde
1
Xuebijing Injection Protocol for Sepsis
2
Chinese Herbal Intervention for Colitis
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All the Chinese herbs were provided by Tongde Hospital of Zhejiang Province (Hangzhou, China). All the chemical standards, including l (+)-arginine, proline, adenine, succinic acid, isoleucine, leucine, gallic acid, phenylalanine, protocatechuic acid, l -tryptophan, protocatechuic aldehyde, neo-mangiferin, chlorogenic acid, caffeic acid, amygdalin, mangiferin, rutin, liquiritin, luteoloside, naringin, berberrubine, baicalin, quercetin, epimedin A, epimedin B, epimedin C, icarrin, isorhamnetin, glycyrrhizic acid, limonin, aaikosaponin A, and 3-N-butyl-4,4-dihydrophthalide, were purchased from Shanghai Yuanye Bio-Technology Co. Ltd (Shanghai, China). Azoxymethane (AOM) was obtained from Sigma-Aldrich (St. Louis, MO, United States) and dextran sodium sulfate (DSS) from MP Biomedicals (Santa Ana, CA United States). TUNEL apoptosis assay kits, radioimmunoprecipitation assay (RIPA) buffer, BCA protein assay kits, and anti-MMP9 antibody (AF5234) were purchased from Beyotime (Beijing, China). Mouse IL-6 and IL-17A ELISA kits were from LiankeBio (Hangzhou, China), and antibodies to p-STAT3 (AF3293), STAT3 (AF6294), NF-κB-p65 (BF8005), Bcl-2 (BF9103), Lamin B (AF5161), and β-actin (T0022) were purchased from Affinity Biosciences (Changzhou, China). Antibodies to IL-17RA (sc-376374) and Bax (sc-20067) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, United States).
3
Protocatechuic Aldehyde Bioassay Protocol
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Protocatechuic aldehyde (purity ≥ 98%) was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). PhosSTOP (4906837001) and EDTA-free, EASYpack protease inhibitor (4693116001) were obtained from Roche (Shanghai, China). Pronase (10165921001) and isoprenaline (I5627) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Trypsin (0.25%), protein A/G magnetic beads (Pierce™, 88802) and Mito-Tracker Red FM (M22425) were all obtained from Thermo Fisher Scientific (Shanghai, China). The H2DCFDA (HY-D0940) fluorescence probe was obtained from MCE (Shanghai, China). PNU-74654 (S8429) was obtained from Selleck (Shanghai, China). Antibodies against PKM2 (4053), SIRT6 (12486), cleaved caspase 3 (9664s), α-actinin (6487), mouse anti-rabbit IgG (light-chain specific, D4W3E) mAb (93702) and the rabbit IgG (2729) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against GAPDH (60004-1-Ig), β-catenin (51067-2-AP, 66379-1-Ig), PCNA (10205-2-AP), caspase 3 (66470-2-Ig), BAX (60267-1-Ig), BCL-2 (60178-1-Ig), PKM2 (60268-1-Ig), β-tubulin (10068-1-AP) and TCF4 (22337-1-AP) were obtained from Proteintech (Wuhan, China). Annexin V-FITC apoptosis detection Kit was obtained from Beyotime (C1062M, Suzhou, China).
4
Chitosan-Based Antioxidant Biomaterials
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Chitosan (medium viscosity: 200–400 mPa s, average molecular weight 50 000–150 000), carrageenan, 1,1-diphenyl-2-picrylhydrazyl (DPPH·), salicylic acid (≥98%), BSA (≥98%), UK (≥85%) ferrous chloride tetrahydrate (≥98%), and 2-phenyl-4,4,5,5-tetramethylimidazoline-3-oxide-1-oxyl (PTIO·) were obtained from Aladdin Reagent Co., Ltd (Shanghai, China). Sodium tripolyphosphate (TPP, ≥98%) was ordered from Shanghai Acmec Biochemical Co., Ltd. Protocatechuic aldehyde (≥98%) and Span 80 were available from Shanghai Yuanye Bio-Technology Co., Ltd, paraffin and petroleum ether were purchased from Shanghai Titan Scientific Co., Ltd. Dulbecco's Modified Eagle Medium (DMEM), Phosphate Buffer Saline (PBS, 1×), fetal bovine serum (FBS), and trypsin–EDTA (0.25% trypsin and 0.913 mM EDTA tetrasodium) were purchased from Gibco (USA). Mouse fibroblast cells (L929) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cell Counting Kit-8 (CCK-8) was brought from Beyotime Biotechnology. A live & dead viability/cytotoxicity assay kit was obtained from YEASEN Inc (Shanghai, China). Sprague Dawley (SD) rats and balb/c mice were supplied by the affiliated hospital of Qingdao university laboratory animal center. Ultrapure water was supplied by Milli-Q Plus System (Millipore Corporation, United States). All reagents were used without further purification.
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