An antibody against GAPDH (MAB374) was from Millipore; Calnexin (ab22595) was from Abcam; Tubulin (DM1A) was from Sigma; CHMP2A (10477–1-AP) was from Proteintech; IST1 (51002–1-AP) was from Proteintech; CHMP7 (16424–1-AP) was from Proteintech; GFP (7.1/13.1) was from Roche; mCherry (ab167453) was from Abcam; HA.11 (16B12) was from (Biolegend); γH2AX (05–636) was from Sigma; 53BP1 (NB100-305) was from (Novus Biologicals). Anti-CDK1 substrate antibodies were from Cell Signaling Technology (9477S and 2325S). Anti-Histone H3 pS10 was from Cell Signaling Technology (9701S). Anti-LEM2 was from Sigma (HPA017340); anti-HSP90 (F8) was from Santa Cruz; anti-Emerin (10351–1-AP) was from Proteintech. Anti-LAP1 (21459–1-AP) was from Proteintech. Alexa conjugated secondary antibodies were from Invitrogen and HRP-conjugated secondary antibodies were from Millipore. IRDye 800 CW (925–32210) and IRDye 680 RD (925–68071) were from LI-COR Biosciences. Anti-peptide antibodies against CHMP7 pSer3 (3891 and 3892) were generated by immunisation of rabbits with KLH-conjugated peptides (MWpSPEREAEAPAGGC) by GenScript Biotech (Netherlands) B.V.
Gfp 7.1 13 1
Manufactured by Roche
The GFP (7.1/13.1) is a laboratory equipment product by Roche. It is a protein used as a fluorescent marker to track and visualize cellular and subcellular processes in living organisms.
Automatically generated - may contain errors
Lab products found in correlation
Anti histone h3 ps10, by Cell Signaling Technology (1 mentions)
γh2ax, by Merck Group (1 mentions)
Anti hsp90 f 8, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated secondary antibody, by Merck Group (1 mentions)
Anti emerin, by Proteintech (1 mentions)
Mcherry ab167453, by Abcam (1 mentions)
53bp1 nb100 305, by Novus Biologicals (1 mentions)
Klh conjugated peptides, by GenScript (1 mentions)
Irdye 800cw, by LI COR (1 mentions)
Irdye 680rd, by LI COR (1 mentions)
Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Calnexin, by Abcam (1 mentions)
C ebpβ c 19, by Santa Cruz Biotechnology (1 mentions)
C ebpδ c 22, by Santa Cruz Biotechnology (1 mentions)
C ebpα 14aa, by Santa Cruz Biotechnology (1 mentions)
Ac 15, by Merck Group (1 mentions)
Trans blot turbo system, by Bio-Rad (1 mentions)
Proteinase inhibitor cocktail, by Roche (1 mentions)
2 protocols using gfp 7.1 13 1
1
Antibody Validation and Characterization
2
Protein Extraction and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein lysates were prepared by incubation of snap-frozen cell pellets with lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% Nonidet P-40, 1 mM EDTA, 10% glycerol, and proteinase inhibitor cocktail [Roche] in H2O) for 30 min on ice. After centrifugation protein concentration was measured in supernatants using Pierce Reagent (Thermo Scientific). Protein lysates were subjected to electrophoresis and transferred to a nitrocellulose membrane on the Trans-Blot Turbo System (Bio-Rad). Specific protein signals were detected by incubation with antibodies against C/EBPα (14AA, Santa Cruz Biotechnology), C/EBPβ (C-19, Santa Cruz), C/EBPδ (C-22, Santa Cruz), C/EBPε (C-22, Santa Cruz), GFP (7.1/13.1, Roche), and ACTB (AC-15, Sigma).
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