Fmoc-protected amino acids were purchased from GL Biochem Ltd (Shanghai, China). HSPC (hydrogenated soy phosphatidylcholine) and mPEG2000-DSPE were obtained from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol was supplied by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Near infrared dye DiR was obtained from Invitrogen, USA. Sephadex G50 and 5-carboxyfluorescein (FAM) were provided by Sigma (St. Louis, MO). Doxorubicin hydrochloride was supplied by Dalian Meilun Biotech Co., Ltd (Dalian, China). Rat tail collagen Type I was purchased from Shengyou Biological Technology Co. (Hangzhou, China). DNase I and collagenase were supplied by Dingguo Biological Technology Co. Ltd (Shanghai, China).
Human glioblastoma cells (U87), human umbilical vascular endothelial cells (HUVECs) and brain capillary endothelial cells (bEnd.3) were obtained from Shanghai Institute of Cell Biology, cultured in special Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco). ICR mice and BALB/c nude mice aged 4-6 weeks were supplied by Shanghai SLAC Laboratory Animal Co. Ltd (Shanghai, China) and housed under SPF conditions. All animal experiments were carried out in accordance with the guidelines evaluated and approved by the Ethics Committee of Fudan University.
Human glioblastoma cells (U87), human umbilical vascular endothelial cells (HUVECs) and brain capillary endothelial cells (bEnd.3) were obtained from Shanghai Institute of Cell Biology, cultured in special Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco). ICR mice and BALB/c nude mice aged 4-6 weeks were supplied by Shanghai SLAC Laboratory Animal Co. Ltd (Shanghai, China) and housed under SPF conditions. All animal experiments were carried out in accordance with the guidelines evaluated and approved by the Ethics Committee of Fudan University.