Cover slips for microscopy

Anesthetic MS-222, water-soluble progesterone, and ATP Bioluminescence Assay Kit CLS II were purchased from Sigma (St. Louis, MO). Collagenase (280 U/mg) was obtained from Wako (Osaka, Japan) and human chorionic gonadotropin was from Teikoku Zoki (Tokyo Japan). Hydrogen peroxide, Sudan Black B (SBB), and protein assay CBB solution were from Nacalai Tesque (Kyoto, Japan). Hydrogen peroxide colorimetric/fluorometric assay kit was from BioVision (Milpitas, CA). Other chemicals were obtained from Wako and Nacalai Tesque. Slide glasses and cover slips for microscopy were purchased from Matsunami Glass (Osaka, Japan).

Water-soluble progesterone (PG), anesthetic MS-222 and ATP Bioluminescence Assay Kit CLS II were purchased from Sigma (St. Louis, MO, USA). hCG was from Teikoku Zoki (Tokyo, Japan) and collagenase (280 U/mg) was obtained from Wako (Osaka, Japan). The hydrogen peroxide colorimetric/fluorometric assay kit was from BioVision (Milpitas, CA, USA). Fluorogenic caspase-3 substrate IV was purchased from Calbiochem (La Jolla, CA, USA). Polyclonal anti-cyclin B2 antibody was ordered from Santa Cruz (Santa Cruz Biotechnology, Dallas, TX, USA), biotinylated anti-rabbit IgG was from Vector Laboratories (Burlingame, CA, USA). The Streptavidin Biotin Complex Peroxidase Kit, protein assay CBB and hydrogen peroxide were from Nacalai Tesque (Kyoto, Japan). MitoTracker Deep Red FM was from ThermoFisher (Waltham, MA, USA). Luciferase control RNA and luciferase assay system were from Promega (Madison, WI, USA). Other chemicals were obtained from Wako and Nacalai Tesque. Slide glasses and cover slips for microscopy were purchased from Matsunami Glass (Osaka, Japan).