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Lmna antibodies

Manufactured by Santa Cruz Biotechnology
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LMNA antibodies are laboratory reagents designed to detect and study the LMNA gene and its associated proteins. LMNA encodes the lamin A and lamin C proteins, which are important structural components of the cell nucleus. These antibodies can be used to identify and analyze the expression and localization of lamin A and lamin C in various cell and tissue samples.

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Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (2 mentions) Chip dna clean and concentrator, by Zymo Research (2 mentions)

2 protocols using lmna antibodies

1

LMNA Chromatin Immunoprecipitation Protocol

Cited 1 time
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LMNA antibodies (Santa Cruz Biotechnology; sc-6215 and sc-7292) was incubated with Dynabeads (Life Technology; 10003D) for 12 hr at 4 °C. A small portion of the crosslinked, sheared chromatin was saved as the Input, and the remainder was employed for the immunoprecipitation using antibody-conjugated Dynabeads. After overnight incubation at 4 °C, the incubated beads ware rinsed with a sonication buffer (50 mM Hepes pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, 0.5 mM PMSF), a high salt buffer (50 mM Hepes pH 7.9 500 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, 0.5 mM PMSF), and a LiCl buffer (20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% NP-40, 0.5% Na-deoxycholate, 0.5 mM PMSF). The washed beads were incubated with an elution buffer (50 mM Tris, pH 8.0, 1 mM EDTA, 1% SDS, 50 mM NaHCO3) for 1 hr at 65 °C and then de-crosslinked with 5 M NaCl overnight at 65 °C. The immunoprecipitated DNA was treated with Rnase A and Proteinase K, and purified by ChIP DNA clean and concentrator (Zymo Research; D5205). The raw sequencing data were analyzed as previously described39 (link).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
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2

LMNA Chromatin Immunoprecipitation Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
LMNA antibodies (Santa Cruz Biotechnology; sc-6215 and sc-7292) was incubated with Dynabeads (Life Technology; 10003D) for 12 hr at 4 °C. A small portion of the crosslinked, sheared chromatin was saved as the Input, and the remainder was employed for the immunoprecipitation using antibody-conjugated Dynabeads. After overnight incubation at 4 °C, the incubated beads ware rinsed with a sonication buffer (50 mM Hepes pH 7.9, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, 0.5 mM PMSF), a high salt buffer (50 mM Hepes pH 7.9 500 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS, 0.5 mM PMSF), and a LiCl buffer (20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% NP-40, 0.5% Na-deoxycholate, 0.5 mM PMSF). The washed beads were incubated with an elution buffer (50 mM Tris, pH 8.0, 1 mM EDTA, 1% SDS, 50 mM NaHCO3) for 1 hr at 65 °C and then de-crosslinked with 5 M NaCl overnight at 65 °C. The immunoprecipitated DNA was treated with Rnase A and Proteinase K, and purified by ChIP DNA clean and concentrator (Zymo Research; D5205). The raw sequencing data were analyzed as previously described39 (link).
Lee J., Termglinchan V., Diecke S., Itzhaki I., Lam C.K., Garg P., Lau E., Greenhaw M., Seeger T., Wu H., Zhang J.Z., Chen X., Gil I.P., Ameen M., Sallam K., Rhee J.W., Churko J., Chaudhary R., Chour T., Wang P.J., Snyder M.P., Chang H.Y., Karakikes I, & Wu J.C. (2019). Activation of PDGF Pathway Links LMNA Mutation to Dilated Cardiomyopathy. Nature, 572(7769), 335-340.
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