Blood was taken from healthy volunteers and collected into VACUETTE® tubes with Serum Clot Activator (ref. 456018, Greiner bio-one), then centrifuged. Serum samples were pooled, aliquoted and stored at −80 °C.
Serum clot activator
Manufactured by Greiner
Sourced in Austria
The Serum Clot Activator is a laboratory equipment designed to facilitate the coagulation process in blood samples. It provides a consistent and controlled environment to activate the clotting cascade, enabling the separation of serum from the clot for further analysis.
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Lab products found in correlation
6 protocols using serum clot activator
1
Serum Sample Preparation from Healthy Volunteers
2
Fibrin Nanofibers Preparation
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The human whole blood was collected in a tube containing serum clot activator (Greiner Bio-One, Kremsmünster, Austria) and centrifuged at 2000× g for 15 min at 25 °C. After centrifugation, serum-containing thrombin was collected and mixed to 10% (w/v) CaCl2 (Sigma-Aldrich, St. Louis, MO, USA) in a 9:1 ratio (serum: Ca2+v/v). This mixture was added to the L-PRP formulations at a concentration of 20% (v/v), which were left to polymerize for at least 30 min to form the fibrin100, fibrin800, fibrin100-400, and fibrin800-400 nanofibers.
3
Serum Sample Collection and Storage
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Blood was taken from healthy volunteers and collected in VACUETTE® tubes with Serum Clot Activator (ref. 456,018, Greiner bio-one), then centrifuged. Serum was pooled, aliquoted and stored at − 80 °C.
4
Assaf Sheep Population Sampling
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According to the latest data from the Palestinian Central Bureau of Statistics, the estimated sheep population in the target cities is 2,391,169, of which the Assaf breed represents 47.3% (113,127) [23 ]. All samples were collected from the Assaf breed. The number of animals sampled from each city was: Jenin (n = 104), Nablus (n = 120), Tulkarm (n = 36), and Qalqilya (n = 20) (Figure-1 ). Data regarding location, age in years (≤1, 1–3, and >3 years), sex, and pregnancy were recorded. After clinical examination, 5 mL of blood was collected from the jugular vein in plain tubes containing a clotting activator (Vacuette, Serum Clot activator, Greiner bio-one, Kremsmunster, Austria) and sent to the Virology Laboratory at the Department of Veterinary Medicine, An-Najah National University for analysis.
5
Serum Lipid Metabolite Analysis
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All blood samples were collected in serum separator tubes (Serum Clot Activator, Greiner Bio-one GmbH, Kremsmunster, Austria), and the samples were allowed to clot for a minimum of 25 min at 20°C and stored in the refrigerator overnight. The samples were then centrifuged at 3,000 × g for 15 min at 4°C before separation of the serum. Nonesterified fatty acid (NEFA), BHB, and triglycerides in the blood were analyzed using commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing Jiancheng Technology Co. Ltd., Nanjing, China) according to the manufacturer's instructions.
6
Blood Metabolite Profiling in Dairy Cows
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Duplicate blood samples of approximately 15-mL were collected via a coccygeal vein from individual cows at 0700 h daily on d -21, -14, -7, 0, 3, 7, 14, and 21 relative to calving. All the blood samples were collected in serum separator tubes (Serum Clot Activator, Greiner Bio-one GmbH, Kremsmunster, Austria), and the samples were allowed to clot for a minimum of 25 min at 20°C and stored in the refrigerator overnight. The samples were then centrifuged at 3,000 × g for 15 min at 4°C before separation of the serum. Blood serum was analyzed for BHB and FFA concentrations using commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing Jiancheng Technology Co. Ltd., Nanjing, China) according to the manufacturer's instructions.
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