Immunofluorescently labelled retina sections were imaged with an ANDOR iXon Life 897 EMCCD camera (Oxford instruments, Abingdon, United Kingdom) on an Olympus BX51WI fluorescence microscope with or without SRRF reconstruction. The loci of Cx36 were identified by the green fluorescence (λEx: 450–490 nm, λEm: 500–550 nm) through a water immersion objective, Olympus LUMPlanFL lens 40x/0.80 N.A. Live cell imaging was conducted with Cx36-GCaMP-transfected HeLa cells superfused with 37°C pre-warmed and oxygenated Ames medium perfused by a peristaltic pump (Model P720, Instech Laboratories, Plymouth Meeting, PA, United States). The GCaMP loci were imaged with a doubler in addition to Olympus LUMPlanFL lens 40x/0.80 N.A. water immersion objective through the bandpass filters (λEx: 490–510 nm, λEm: 520–550 nm) for quantitatively measuring the effect of transient ionomycin puffing on GCaMP fluorescence.
Lumplanfl lens 40x 0.80 n a
Manufactured by Olympus
Sourced in United States
The LUMPlanFL lens 40x/0.80 N.A. is a high-performance objective lens designed for use in microscopy applications. It features a magnification of 40x and a numerical aperture (N.A.) of 0.80, which allows for efficient light collection and high-resolution imaging.
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2 protocols using lumplanfl lens 40x 0.80 n a
1
Cx36 and GCaMP Fluorescence Imaging
2
Cx36 and GCaMP Fluorescence Imaging
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Immunofluorescently labelled retina sections were imaged with an ANDOR iXon Life 897 EMCCD camera (Oxford instruments, Abingdon, United Kingdom) on an Olympus BX51WI fluorescence microscope with or without SRRF reconstruction. The loci of Cx36 were identified by the green fluorescence (λEx: 450–490 nm, λEm: 500–550 nm) through a water immersion objective, Olympus LUMPlanFL lens 40x/0.80 N.A. Live cell imaging was conducted with Cx36-GCaMP-transfected HeLa cells superfused with 37°C pre-warmed and oxygenated Ames medium perfused by a peristaltic pump (Model P720, Instech Laboratories, Plymouth Meeting, PA, United States). The GCaMP loci were imaged with a doubler in addition to Olympus LUMPlanFL lens 40x/0.80 N.A. water immersion objective through the bandpass filters (λEx: 490–510 nm, λEm: 520–550 nm) for quantitatively measuring the effect of transient ionomycin puffing on GCaMP fluorescence.
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