Whatman filter paper grade 41 and grade 1

Materials include the following: Whatman filter paper grade #41 and grade #1 (GE Healthcare Life Sciences), gold (III) chloride hydrate (99.9%, Sigma-Aldrich), sodium citrate tribasic dihydrate (99%, Sigma-Aldrich), 30% poly(sodium 4-styrenesulfonate) solution (PSS, Sigma-Aldrich), bovine serum albumin (BSA ≥ 98%, VWR Chemicals), sucrose (99.5%, Sigma-Aldrich), Tween 20 (Sigma-Aldrich), 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (NHS-FL 75%, Sigma-Aldrich), pyocyanin (98%, Sigma-Aldrich), high affinity mouse monoclonal IgG antibodies anti-pyocyanin (mAb122) produced in-house [16 (link)], PC1-BSA bioconjugate (produced in-house, hapten density = 10), goat anti-mouse-IgG/HRP (Dako Agilent), sodium bicarbonate (99.7%, Sigma-Aldrich), sodium carbonate (≥ 97%, VWR Chemicals), 30% hydrogen peroxide solution (Sigma-Aldrich), 3,3′,5,5′-tetramethylbenzidine (TMB, 99%, Thermo Fisher), sulfuric acid (95%, VWR Chemicals), Bradford reagent (VWR Chemicals), PD-10 Desalting Column (GE Healthcare), 96-well maxisorp ELISA microplates (Thermo Scientific). PBS refers to phosphate buffered saline (pH 7.4), PBST refers to PBS with 0.1% Tween 20, and PBS-BSA refers to PBS containing 5 mg·mL⁻¹ BSA).

Materials include the following: Whatman filter paper grade #41 and grade #1 (GE Healthcare Life Sciences), gold (III) chloride hydrate (99.9%, Sigma-Aldrich), sodium citrate tribasic dihydrate (99%, Sigma-Aldrich), 30% poly(sodium 4-styrenesulfonate) solution (PSS, Sigma-Aldrich), bovine serum albumin (BSA ≥ 98%, VWR Chemicals), sucrose (99.5%, Sigma-Aldrich), Tween 20 (Sigma-Aldrich), 6-[Fluorescein-5(6)-carboxamido] hexanoic acid N-hydroxysuccinimide ester (NHS-FL 75%, Sigma-Aldrich), pyocyanin (98%, Sigma-Aldrich), high affinity mouse monoclonal IgG antibodies anti-pyocyanin (mAb122) produced in-house [16 (link)], PC1-BSA bioconjugate (produced in-house, hapten density = 10), goat anti-mouse-IgG/HRP (Dako Agilent), sodium bicarbonate (99.7%, Sigma-Aldrich), sodium carbonate (≥ 97%, VWR Chemicals), 30% hydrogen peroxide solution (Sigma-Aldrich), 3,3′,5,5′-tetramethylbenzidine (TMB, 99%, Thermo Fisher), sulfuric acid (95%, VWR Chemicals), Bradford reagent (VWR Chemicals), PD-10 Desalting Column (GE Healthcare), 96-well maxisorp ELISA microplates (Thermo Scientific). PBS refers to phosphate buffered saline (pH 7.4), PBST refers to PBS with 0.1% Tween 20, and PBS-BSA refers to PBS containing 5 mg·mL⁻¹ BSA).