Ir affinity 1s infrared spectrophotometer

Melting points (m.p.) were determined on the Mettler Toledo-MP90 Melting Point System and were uncorrected. IR spectra were recorded on an IR Affinity-1S Infrared spectrophotometer (Shimadzu, Tokyo, Japan). ¹H NMR and ¹³C NMR spectra in DMSO-d₆ were recorded on a Bruker Fourier 300 (Bruker Bioscience, Billerica, MA, USA), respectively. MS studies were performed on an LCMS-8040 tandem mass system (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F254 (Merck KGaA, Darmstadt, Germany). The Rf values of the synthesized compounds were measured using the solution system of petroleum ether:ethyl acetate (1:1).

All reagents were purchased from commercial suppliers and were used without further purification. Prominence HPLC system (Shimadzu, Kyoto, Japan), equipped with a Shimadzu DGU-14 A degasser, LC-20 A dual piston pump, CTO-10 ASVP column oven and SPD-MI20A PDA detector Sıl-20ACXR autosampler and a stainless-steel GL Science Inertsil ODS-3 (4.6 250 mm) column. Solvents (acetonitrile and water, 7:3) for the separation obtained compounds were isocritically mixed at a flow rate of 0.25 mL/min and injection were performed by volume of 10 µL. IR spectra was recorded on an IR Affinity-1S Infrared spectrophotometer (Shimadzu, Tokyo, Japan). Melting points (M.p.) were determined using the Mettler Toledo-MP90 Melting Point System and were uncorrected. ¹H NMR and ¹³C NMR spectra was recorded on a Bruker Fourier 300 (Bruker Bioscience, Billerica, MA, USA) respectively, in DMSO-d₆. (Bruker, Billerica, MA, USA), respectively. MS studies were performed on an LCMS-8040 tandem mass system (Shimadzu, Tokyo, Japan).