Lumera 700

A standard ophthalmic surgery microscope equipped with an OCT engine (LUMERA 700 with RESCAN 700, Carl Zeiss Meditec, München, Germany) was used for all experiments. Two OCT B-scans in a cross formation were continuously captured by the microscope at a desired location controlled by the surgeon using a foot control pedal or by the assistant using the auxiliary screen. The surgical field was directly observed through the microscope’s eyepiece with the two B-scans and an OCT acquisition location marker overlaid onto the scene.

This retrospective study included 23 eyes of 23 patients. A standard 3-port pars plana vitrectomy with a Chandelier light source was performed using the Constellation 23- and 25-gauge vitrectomy system (Alcon Laboratories Inc, Fort Worth, TX, USA). After vitrectomy, the posterior vitreoretinal interface was visualized by iOCT (OPMI LUMERA^® 700, ZEISS^© with integrated OCT). The ILM was stained using 0.1 mL of Brilliant Blue G (Brilliant Peel; Geuder, Germany) for approximately 1 min after stopping infusion and then excess dye was removed. iOCT was used to identify small discontinuities/gaps between the ERM and the nerve fiber layer (Figure 2), which were used as a scaffold to initiate membrane removal in a targeted manner with minimal nerve fiber layer disturbance. The ILM was grasped with end-gripping forceps at a thick or wrinkled spot and a horizontal ILM strip was peeled off (Figure 3). Fluid–air exchange was performed using a 23- and 25-gauge flute needle held nasal to the disk. The vitreous cavity was then filled either with air or 25% perfluoropropane gas.