Ascorbic 3 phosphate

Manufactured by Merck Group

Sourced in United States

Ascorbic 3-phosphate is a laboratory reagent that serves as a source of ascorbic acid (vitamin C) in biochemical and cell culture applications. It is a water-soluble compound that can be used to supplement cell growth media or to provide a stable form of ascorbic acid for various experimental purposes.

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Lab products found in correlation

Dexamethasone (dex), by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Bovine serum, by Thermo Fisher Scientific (1 mentions) β glycerol phosphate, by Merck Group (1 mentions) Tgf β3, by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions) Bovin serum albumin, by Merck Group (1 mentions) Na β glycerophosphate, by Merck Group (1 mentions) Ascorbic 2 phosphate, by Merck Group (1 mentions)

Spelling variants of this product

Ascorbic acid 3-phosphate (6 citations) L-ascorbic-3-phosphate (1 citations)

2 protocols using ascorbic 3 phosphate

Osteogenic Differentiation of Transduced Cells

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Cells were grown in a six-cell plate when the entire culture surface was covered with
cells, and their media changed with a bone differentiation medium to generate osteogenic
lineage cells as a mesodermal lineage in transduced cells. This medium included Dulbecco’s
Modified Eagle Medium (DMEM, Gibco, UK) with 10% bovine serum (Gibco, UK), 10 mM beta
glycerol phosphate (Sigma-Aldrich, USA), 10 nM dexamethasone (Sigma-Aldrich, USA), and 50
g/ml ascorbic 3-phosphate (Sigma-Aldrich, USA), The cells were then put on mesenchymal
cells by using a sampler. For 21 days, cells were cultured in a humidified 37°C incubator
with 5% CO₂ . Finally, immunocytochemistry was performed to validate the
differentiation of cells using the alkaline phosphatase marker (Santa Cruz, USA) (19 (link)).

Hasani Fard A.H., Kamalipour F., Mazaheri Z, & Hosseini S.J. (2022). The Effect of miR-106b-5p Expression in The Production of iPS-Like Cells from Mice SSCs during The Formation of Teratoma and The Three Embryonic Layers. Cell Journal (Yakhteh), 24(8), 442-448.

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Differentiation of Mesenchymal Stem Cells

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Alizarin red staining and oil red staining were used for passage 3 cMSCs cells cultured for 3 weeks in the osteogenic, adipogenic, and chondrogenic differentiation mediums, respectively. Mediums were changed twice per week. The osteogenic differentiation medium contained the control medium supplemented with 0.2 mM ascorbic2phosphate (Sigma, St. Louis, MO), 10 mM Na-b-glycerophosphate (Sigma Co.), and 10 -8 M dexamethasone (Sigma). The adipogenic differentiation medium contained the control medium supplemented with 0.2 mM ascorbic3 phosphate (Sigma, St. Louis, MO), 100 μM indomethacin (Sigma Co.), and 10 -5 M dexamethasone (Sigma). The chondrogenic differentiation medium contained the control medium supplemented with 10 ng TGF-β3 (Transforming Growth Factor-β3, Sigma), 10 ng BMP-6(Bone Morphogenetic protein-6, sigma Co.), 50 ng ITS + premix (Insulin-Transferin-Selenium, Sigma) and 5.35 mg Bovin Serum Albumin (Sigma). After 3 weeks, cell layers were washed twice with PBS, fixed with 4% formalin for 15 min, and then washed with distilled water. Subsequently, cells were incubated for 2 min with either alizarin red or oil red, and evaluated by light microscope.

Khojasteh A., Fahimipour F., Eslaminejad M.B., Jafarian M., Jahangir S., Bastami F., Tahriri M., Karkhaneh A, & Tayebi L. (2016). Development of PLGA-coated β-TCP scaffolds containing VEGF for bone tissue engineering. Materials science & engineering. C, Materials for biological applications, 69.

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