The expression of surface markers on MSC, nvMSC-HLA-G1, and vMSC-HLA-G1 was evaluated by flow cytometry using the following monoclonal anti-human antibodies: HLA-G FITC (ABD Serotec, Oxford, UK), HLA DR, DP, DQ FITC (BD Bioscience, San Jose, CA), HLA-ABC PE (BD Pharmingen, San Jose, CA), MICA/MICB FITC, CD155 FITC (both from ABD Serotec), ULBP-1 PE, ULBP-2 PE (both from R&D Systems), CD58 PE (BD Biosciences), TLR9 FITC, TLR4 PE (both from Abcam, Cambridge, UK) and TLR3 Alexa 647 (Imagenex, Port Coquitlam, BC, Canada). Cells were also stained for HLA E (Abcam), ULBP-3 (R&D Systems), and CD112 (ABD Serotec) using Alexa Fluor 488 Goat Anti-Mouse IgG (Molecular Probes-Life Technologies) as secondary antibody. Briefly, cells were incubated for 15 minutes in the dark with saturating concentrations of each antibody. Stained cells were then washed with phosphate-buffered saline 0.1% azide, fixed with 1% formaldehyde, and analyzed by flow cytometry using the CellQuest software (Becton Dickinson Biosciences, San Jose, CA). A total of 10,000 events was acquired for each sample. Appropriate isotype (negative) controls were performed for each antibody.
Cd58 pe
Manufactured by BD
Sourced in United Kingdom
The CD58 PE is a fluorescent-labeled antibody that can be used to identify and quantify CD58-expressing cells in flow cytometry applications. CD58 is a cell surface glycoprotein that functions as a ligand for the T-cell co-stimulatory receptor CD2. The PE (Phycoerythrin) fluorescent label allows for detection and analysis of CD58-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions)
Cellquest software, by BD (2 mentions)
Ulbp3, by R&D Systems (2 mentions)
Ulbp1 pe, by R&D Systems (2 mentions)
Hla abc pe, by BD (2 mentions)
Hla g fitc, by Bio-Rad (2 mentions)
Mica micb fitc, by Bio-Rad (2 mentions)
Cd155 fitc, by Bio-Rad (2 mentions)
Ulbp 2 pe, by R&D Systems (2 mentions)
Tlr9 fitc, by Abcam (2 mentions)
Tlr4 pe, by Abcam (2 mentions)
Hla e, by Abcam (2 mentions)
Cd112, by Bio-Rad (2 mentions)
Cd66 pe, by BD (1 mentions)
Cd54 pe, by BD (1 mentions)
Cd71 pe, by BD (1 mentions)
Cd44 apc cy7, by BD (1 mentions)
Cd117 pe, by BD (1 mentions)
Cd24 pe cy7, by BD (1 mentions)
Cd10 pecf594, by BD (1 mentions)
3 protocols using cd58 pe
1
Evaluating Surface Marker Expression in MSC Variants
2
Multiparameter Flow Cytometry of CTCs
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CTCs from cells suspension were characterized by multiparameter flow cytometry. The antibodies used in this study include: anti-human CD133-APC, CD44-FITC, CD44-APC-Cy7, CD54-PErcp-cy5.5, CD54-PE, CD24-PE/Cy7, CD10-PECF594, CD26-PE, CD166-Percp-cy5.5, CD45-BV510, CD58-PE, CD66-PE, CD71-PE, CD117-PE, EPCAM-Percp-cy5.5, and EGFR-PE (all of the above-mentioned antibodies were purchased from BD Biosciences). DAPI was used to identify the dead cells. Evaluation of nucleated cells from whole cells suspensions was carried out using a FACS Canto Flow Cytometer (BD Biosciences) and data were analyzed using BD FACS Diva software. A range of internal quality assurance procedures was employed, including daily calibration of the optical alignment and fluidic stability of the flow cytometer using the seven-color Set-up Beads (BD Biosciences). The absolute CTCs or antibody-positive cell number was derived from the absolute number of the white blood cells provided by the hematological analyzer and percentage of CTCs or antibody-positive cell as determined by flow cytometry, using the following formula: percentage of cells × white blood cells count/100.
3
Evaluating Surface Marker Expression in MSC Variants
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