For TUNEL assay, MCF7 and T47D cells were cultured on glass coverslips. Cells were fixed, permeabilized and TUNEL assay was performed using the ApopTag Red detection kit (Chemicon, Inc.) following manufacturer's protocol. Samples were counterstained with DAPI before mounting. Cells were visualized using fluorescent microscopy.
Apoptag red detection kit
Manufactured by Merck Group
The ApopTag Red detection kit is a laboratory product designed to detect and visualize apoptosis, a form of programmed cell death, in cell samples. The kit utilizes an enzymatic process to label and identify DNA fragmentation, a hallmark of apoptosis. The resulting red-colored signal allows for the quantification and analysis of apoptotic cells.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using apoptag red detection kit
1
Apoptosis Detection in Breast Cancer Cells
2
Apoptosis Detection in Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For TUNEL assay, MCF7 and T47D cells were cultured on glass coverslips. Cells were fixed, permeabilized and TUNEL assay was performed using the ApopTag Red detection kit (Chemicon, Inc.) following manufacturer's protocol. Samples were counterstained with DAPI before mounting. Cells were visualized using fluorescent microscopy.
3
TUNEL Assay and MPO Activity Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TUNEL assay was performed using ApopTag Red detection kit (Millipore) according to manufacturer’s protocol. Four randomly selected high-power microscopic fields of each sample were examined using Leica DM 4000B microscope mounted with QI Click digital CCD camera. Number of TUNEL-positive cells were scored and normalized by total number of cells. MPO activity was measured as previous described50 (link). Enzymatic activity was measured at 450 nm using 96-well Multiskan microplate reader and determined from a standard curve generated by a serial dilution of purified MPO enzyme (Enzo Life Sciences).
4
TUNEL Assay and MPO Activity Quantification
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!