T cell expansion media

Example 7

CD8+ T cells were isolated from human PBMCs using negative magnetic bead selection (Stemcell Technologies) and cultured in T-Cell Expansion Media (StemCell). T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Invitrogen) and 30 ng/mL recombinant human IL-2 (Peprotech) or left unactivated.

CD8+ T cells in culture were found to produce serotonin, as detected by ELISA (Novus Biologicals). When CD8+ T cells were activated, serotonin levels dropped. Because there was no other cellular source of serotonin in the culture, it seems that CD8+ T cells actively produced and released serotonin.

Serotonin levels in the cultures of human CD8+ T cells that were activated or left unactivated are shown in Table 21 below.

Example 6

CD8+ T cells were isolated from human PBMCs using negative magnetic bead selection (Stemcell Technologies) and cultured in T-Cell Expansion Media (StemCell). T cells were activated with Dynabeads Human T-Activator CD3/CD28 (Invitrogen) and 30 ng/mL recombinant human IL-2 (Peprotech) or left unactivated.

CD8+ T cells in culture were found to produce serotonin, as detected by ELISA (Novus Biologicals). When CD8+ T cells were activated, serotonin levels dropped. Because there was no other cellular source of serotonin in the culture, it seems that CD8+ T cells actively produced and released serotonin.

Serotonin levels in the cultures of human CD8+ T cells that were activated or left unactivated are shown in Table 19 below.