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8050 triple quadrupole mass spectrometer

Manufactured by Phenomenex
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The 8050 triple quadrupole mass spectrometer is a high-performance analytical instrument designed for precise and sensitive detection and quantification of chemical compounds. It utilizes three quadrupole mass analyzers to perform tandem mass spectrometry (MS/MS) analysis, enabling the identification and measurement of target analytes with high specificity and accuracy.

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Lab products found in correlation

Luna 5 μm silica column, by Phenomenex (2 mentions)

2 protocols using 8050 triple quadrupole mass spectrometer

1

Targeted LC-MS/MS Analysis of Carnitine Metabolites

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Plasma levels of endogenous and stable isotope–labeled L-carnitine, γBB, and TMAO were determined by stable isotope dilution liquid chromatography–tandem mass spectrometry (LC-MS/MS) in positive multiple reaction monitoring (MRM) mode as previously described using a Shimadzu 8050 triple quadrupole mass spectrometer with ultra-HPLC interface as previously described.21 (link) A Luna® 5 μm Silica column (150 × 2 mm, Phenomenex) was used to resolve analytes. The retention time of each analyte was determined by authentic standard. The parent to daughter transitions monitored were mass-to-charge ratio (m/z): m/z 165→63 for d3-L-carnitine; m/z 171→69 for d9-L-carnitine; m/z 149 →63 for d3-γBB; m/z 155→69 for d9-γBB; m/z 63 →47 for d3-TMA; m/z 69 →49 for d9-TMA; m/z 108 →60 for d4-choline; m/z 64 →47 for [13C3,15N]TMA. Laboratory personnel performing MS analyses were blinded to sample group allocation and clinical data during analysis.
Buffa J.A., Romano K.A., Copeland M.F., Cody D.B., Zhu W., Galvez R., Fu X., Ward K., Ferrell M., Dai H.J., Skye S., Hu P., Li L., Parlov M., McMillan A., Wei X., Nemet I., Koeth R.A., Li X.S., Wang Z., Sangwan N., Hajjar A.M., Dwidar M., Weeks T.L., Bergeron N., Krauss R.M., Tang W.H., Rey F.E., DiDonato J.A., Gogonea V., Gerberick G.F., Garcia-Garcia J.C, & Hazen S.L. (2021). The microbial gbu gene cluster links cardiovascular disease risk associated with red meat consumption to microbiota L-carnitine catabolism. Nature microbiology, 7(1), 73-86.
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2

Targeted LC-MS/MS Analysis of Carnitine Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma levels of endogenous and stable isotope–labeled L-carnitine, γBB, and TMAO were determined by stable isotope dilution liquid chromatography–tandem mass spectrometry (LC-MS/MS) in positive multiple reaction monitoring (MRM) mode as previously described using a Shimadzu 8050 triple quadrupole mass spectrometer with ultra-HPLC interface as previously described.21 (link) A Luna® 5 μm Silica column (150 × 2 mm, Phenomenex) was used to resolve analytes. The retention time of each analyte was determined by authentic standard. The parent to daughter transitions monitored were mass-to-charge ratio (m/z): m/z 165→63 for d3-L-carnitine; m/z 171→69 for d9-L-carnitine; m/z 149 →63 for d3-γBB; m/z 155→69 for d9-γBB; m/z 63 →47 for d3-TMA; m/z 69 →49 for d9-TMA; m/z 108 →60 for d4-choline; m/z 64 →47 for [13C3,15N]TMA. Laboratory personnel performing MS analyses were blinded to sample group allocation and clinical data during analysis.
Buffa J.A., Romano K.A., Copeland M.F., Cody D.B., Zhu W., Galvez R., Fu X., Ward K., Ferrell M., Dai H.J., Skye S., Hu P., Li L., Parlov M., McMillan A., Wei X., Nemet I., Koeth R.A., Li X.S., Wang Z., Sangwan N., Hajjar A.M., Dwidar M., Weeks T.L., Bergeron N., Krauss R.M., Tang W.H., Rey F.E., DiDonato J.A., Gogonea V., Gerberick G.F., Garcia-Garcia J.C, & Hazen S.L. (2021). The microbial gbu gene cluster links cardiovascular disease risk associated with red meat consumption to microbiota L-carnitine catabolism. Nature microbiology, 7(1), 73-86.
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