Latrunculin

Manufactured by Merck Group

Latrunculin is a chemical compound that is commonly used in biological research. It functions as an actin polymerization inhibitor, preventing the formation of actin filaments within cells. This disrupts the cellular cytoskeleton, which is essential for various cellular processes. Latrunculin is a useful tool for researchers investigating the role of the actin cytoskeleton in cell biology.

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Lab products found in correlation

Nystatin, by Merck Group (2 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Blebbistatin, by Merck Group (1 mentions) Minimum essential medium (mem), by Corning (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Gm6001, by Merck Group (1 mentions) Ethylisopropyl amiloride, by Merck Group (1 mentions) Vinblastine, by Merck Group (1 mentions) Dansylcadaverine, by Merck Group (1 mentions) 2 bromopalmitate, by Merck Group (1 mentions) Recombinant egf, by Merck Group (1 mentions) Streptavidin sepharose high performance bead, by GE Healthcare (1 mentions) Hcc1806, by Corning (1 mentions) Azido homoalanine, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 media, by Corning (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions)

Close spelling variants of this product

Latrunculin A (187 citations) Latrunculin B (130 citations) Latrunculin-A (LatA, (15 citations) Latrunculin B (Lat-B; (10 citations) Latrunculin B (L5288) (3 citations) Latrunculin A (L5163) (2 citations)

5 protocols using latrunculin

Culturing Cell Lines for Cytoskeletal Assays

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CAFs were cultured in a-modified essential medium (MEM) (Cellgro) supplemented with 20% fetal bovine serum, penicillin/streptomycin, L-glutamine, and sodium pyruvate. Primary cells used in experiments had been passaged fewer than 6 times and were not tested for identity. The 344SQ cell line was cultured at 37°C and 5% CO₂ humidified atmosphere in RPMI 1640 (Mediatech) supplemented with 10% fetal bovine serum (Sigma), penicillin (100 U/ml) and streptomycin (100 µg/ml) (Sigma). Cells were treated with 20 µM GM6001 (EMD Millipore), 100 nM latrunculin (Sigma), or 25 µM blebbistatin (Sigma). 344SQ identity was confirmed by PCR analysis of known somatic mutations three month prior to performing experiments.

Pankova D., Chen Y., Terajima M., Schliekelman M.J., Baird B.N., Fahrenholtz M., Sun L., Gill B.J., Vadakkan T.J., Kim M.P., Ahn Y.H., Roybal J.D., Liu X., Parra Cuentas E.R., Rodriguez J., Wistuba I.I., Creighton C.J., Gibbons D.L., Hicks J.M., Dickinson M.E., West J.L., Grande-Allen K.J., Hanash S.M., Yamauchi M, & Kurie J.M. (2015). Cancer-associated Fibroblasts Induce a Collagen Cross-link Switch in Tumor Stroma. Molecular cancer research : MCR, 14(3), 287-295.

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Splenocyte Stimulation and Actin Dynamics

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Splenocytes were isolated and plated in 100μl of culture media. Kinase inhibitors Dasatinib, Src-Inhibitor 1, or 1uM Latrunculin were added for 1 hour before stimulation (Sigma) where indicated. Splenocytes were stimulated with 100μl 10mM peroxide or left unstimulated for the times indicated. Cells were fixed with 4% Paraformaldehyde for 20 mins. They were stained with Rhodamine Phalloidin and B cell markers (B220, CD21, CD23) in PBS containing 0.1% Triton X-100, 0.1% azide and 3% FCS and analyzed by flow cytometry.

Wilson C.S., Elizer S.K., Marshall A.F., Stocks B.T, & Moore D.J. (2015). The Regulation of B Lymphocyte Responses to TLR Ligation During Diabetes Prevention in NOD Mice. Journal of diabetes, 8(1), 120-131.

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Cell Line Maintenance and Reagent Sourcing

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All cell lines used were obtained from ATCC (Manassas, VA) and have since been validated (Promega). DU145 (prostate carcinoma), H1993 (lung carcinoma), AU565, and HCC1806 (breast carcinoma) cells were maintained in RPMI 1640 media (Cellgro, Herndon, VA), MDA-MB-231 (breast carcinoma) cells were maintained in DMEM (Cellgro), and HEK293 cells in EMEM (Cellgro); each containing 10% fetal bovine serum (FBS) (Gemini, West Sacramento, CA). Cells were kept at 37°C with 5.0% CO₂. Monensin and brefeldin A were obtained from Biolegend (San Diego, CA), Y27637 from Stem Cell Technology (Vancouver, BC), C75, 2-hydroxymyristic acid, and 17-Octadecynoic Acid from Caymen Chemicals (Ann Arbor, MI), cycloheximide, recombinant EGF, geranylgeranyltransferase inhibitor, farnesyltransferase inhibitor, vinblastine, latrunculin, dansylcadaverine, ethylisopropyl amiloride, methyl-β-cyclodextrin, nystatin, and 2-bromopalmitate were obtained from Sigma-Aldrich (St. Louis, MO). Click-iT® reaction buffers, azido-homoalanine, azide-palmitate, and methionine-free RPMI, were obtained from Invitrogen (Carlsbad, CA). Recombinant HGF was obtained from EMD Millipore (Darmstadt, Germany). Streptavidin Sepharose High Performance beads were obtained from GE Healthcare (Pittsburgh, PA).

Coleman D.T., Gray A.L., Kridel S.J, & Cardelli J.A. (2016). Palmitoylation regulates the intracellular trafficking and stability of c-Met. Oncotarget, 7(22), 32664-32677.

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Pharmacological Modulation of Synaptic Transmission

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D,L-APV (APV, 200, Sigma), picrotoxin (PTX, 50, Sigma),
tetrodotoxin (TTX, 0.5, Millipore), strychnine (STR, 1, Sigma), glycine
(Gly, 400, Sigma), Jasplakinolide (Jasp, 0.5, Invitrogen), Latrunculin A
(Lat A, 10, Sigma), KN-93 (10, Millipore), KN-92 (10, Millipore),
Mdivi-1 (10, Sigma), and CGP52432 (2, Tocris). For reagents solubilized
in DMSO (Sigma), the final amount of DMSO was ≤0.1% by volume.
MNI-Glutamate (Tocris) was prepared to a final concentration of 2 mM
from single-use stock aliquots.

Divakaruni S.S., Van Dyke A.M., Chandra R., LeGates T.A., Contreras M., Dharmasri P.A., Higgs H.N., Lobo M.K., Thompson S.M, & Blanpied T.A. (2018). Long-term potentiation requires a rapid burst of dendritic mitochondrial fission during induction. Neuron, 100(4), 860-875.e7.

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Endocytic Inhibitors and Fluorescent Cargos

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Endocytotic inhibitors such as chlorpromazine, wortmannin, nystatin, dynasore, AS-604850, cytochalasin B, latrunculin and EIPA were purchased from Sigma Aldrich. Pitstop2 and iminodyn-22 was purchased from Abcam. Ammonium chloride (NH₄Cl) was obtained from Merck. A furin-specific inhibitor, decanoyl-L-arginyl-L-valyl-L-lysyl-L-arginyl-chloromethylketone (decRRVKR-CMK), was obtained from Calbiochem. The fluorescently labeled endocytic cargos Cholera toxin B-FITC (CtxB-FITC), Dextran-FITC, Dextran-TxRd, and LysotrackerGreen were purchased from Life Technologies.

Ayala-Nunez N.V., Hoornweg T.E., van de Pol D.P., Sjollema K.A., Flipse J., van der Schaar H.M, & Smit J.M. (2016). How antibodies alter the cell entry pathway of dengue virus particles in macrophages. Scientific Reports, 6, 28768.

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