All chemicals were purchased from Sigma-Aldrich unless specified otherwise. LPS was purchased from InvivoGen. Usp13 inhibitor (Usp13i) and deubiquitinate enzyme inhibitor (DUBi, PR-619) were purchased from Selleckchem. HRP-conjugated anti-mouse and anti-rabbit IgG secondary antibodies, IRAK1, pTAK1, TAK1, K63 polyubiquitin, and K48 polyubiquitin antibodies were purchased from Cell Signaling Technology. β-actin, pIRAK1, and pA20 antibodies were purchased from Thermo Fisher Scientific. Antibodies against IL-1R1, TRAF6, Ubc13, A20, and anti-goat IgG secondary antibodies were purchased from Santa Cruz Biotechnology. The IL-1β antibody was purchased from R&D Systems. Lambda protein phosphatase (PP) was purchased from New England Biolabs.
Anti goat igg secondary antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-goat IgG secondary antibodies are immunoglobulin G (IgG) molecules that bind specifically to goat primary antibodies. They are designed for use in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to detect and amplify the signal from target antigens recognized by goat primary antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Irak1, by Cell Signaling Technology (1 mentions)
β actin, by Thermo Fisher Scientific (1 mentions)
P tak1, by Cell Signaling Technology (1 mentions)
Lambda protein phosphatase, by New England Biolabs (1 mentions)
Il 1β antibody, by R&D Systems (1 mentions)
Lipopolysaccharides (lps), by InvivoGen (1 mentions)
Electrochemiluminescence kit, by Cytiva (1 mentions)
Scion image, by Techcomp Instruments (1 mentions)
Anti sod, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti goat igg secondary antibodies
1
Dissecting IL-1β Signaling Pathway
2
Quantification of Liver Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytoplasmic and nuclear extracts were prepared from liver homogenates[39 (link)]. The supernatant fraction was collected, aliquoted, and stored at −80 °C. Protein content was determined by Bradford’s method[32 (link)]. Lysed proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes[40 (link),41 (link)]. The membranes were then blocked with 5% skim milk in Tris buffer containing 0.05% Tween (TTBS) for 1 hour at room temperature and probed overnight at 4 °C with anti-Nrf2 (57 kDa), anti-Keap1 (69 kDa), anti-NQO1 (31 kDa), anti-SOD (32 kDa), anti-TLR4 (95 to 120 kDa), anti-NFκB (65 kDa), anti-COX-2 (21 kDa), and anti-iNOS (120 kDa) antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, United Staes), diluted 1:200 to 1:1000 with TTBS in dehydrated milk at 5%. Primary antibodies were detected with HRP-conjugated anti-rat IgG, anti-rabbit IgG, or anti-goat IgG secondary antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, United States). Protein detection was performed with a commercially available electrochemiluminescence kit (Amersham Pharmacia Biotech, Little Chalfont, Bucks, England)[42 (link)]. Density of the specific bands was quantified with imaging densitometry software (Scion Image, Scion Corporation, Frederick, MA, United States).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!