An animal model of pathologic bone destruction was generated as described previously.40 (link) In brief, LPS (12.5 mg·kg–1) or PBS was injected into the subcutaneous tissue of nine-week-old male C57BL/6 mouse calvaria. The mice were simultaneously subcutaneously injected with TEPP46 (50 mg·kg–1) or DMSO, followed by an additional injection for three days. The mice were then euthanized five days after LPS injection, and serum samples were collected to measure TNF-α (Cat. # 555268, BD Biosciences), IL-6 (Cat. # 555240, BD Biosciences), and IL-17AF (Cat. # 88-8711, Invitrogen) by ELISA. Calvaria were harvested, fixed, and subjected to μCT analysis followed by histomorphometric analysis.
Il 17af
Manufactured by Thermo Fisher Scientific
Sourced in United States
IL-17AF is a laboratory reagent used in scientific research. It is a recombinant protein that represents the heterodimeric cytokine formed by the IL-17A and IL-17F subunits. The core function of IL-17AF is to support studies of the IL-17 signaling pathway and its role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 6 (il 6), by BD (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Il 12p70, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by Thermo Fisher Scientific (1 mentions)
Il 13, by Thermo Fisher Scientific (1 mentions)
Interleukin 4 (il 4), by BD (1 mentions)
Tumor necrosis factor (tnf), by BD (1 mentions)
Il 10, by BD (1 mentions)
Ifn γ, by BD (1 mentions)
Il 12p70, by BD (1 mentions)
Il 10, by Thermo Fisher Scientific (1 mentions)
Il 23, by Thermo Fisher Scientific (1 mentions)
Dickkopf 1 dkk 1, by R&D Systems (1 mentions)
I fabp, by Hycult Biotech (1 mentions)
Streptavidin peroxidase conjugate, by Jackson ImmunoResearch (1 mentions)
Il 22, by Abcam (1 mentions)
O phenylenediamine dihydrochloride opd, by Merck Group (1 mentions)
4 protocols using il 17af
1
Pathological Bone Destruction Modeling
2
Cytokine Profiling of Mtb-Infected Mice
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Single cells from the lungs and spleens of Mtb-infected or immunized mice were stimulated with early secreted antigenic targets of 6 kDa (ESAT-6) for 12 hours at 37 °C. A sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect TNF-α, IFN-γ, IL-6, IL-1β, IL-12p70, IL-10, and IL-17AF (Invitrogen, San Diego, CA, USA).
3
Cytokine Production in Lung Cells
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Supernatants of lung cell cultures were collected at 24 h and assayed for TNF, IL-10, IL-4, IFN-γ, IL-12p70 (BD Bioscience, San Diego, CA, USA), IL-13 (Biosource, Camarillo, CA, USA) and IL-17AF (e-Bioscience, Invitrogen, ThermoFisher scientific, San Diego, CA, USA) production by sandwich ELISA according to the manufacturers’ instructions supplied with the cytokine-specific kits.
4
Biomarker Profiling in Samples
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The concentrations of tested factors in collected samples were measured using commercially available ELISA kits specific for: 1) interleukin (IL)-10, IL-17 A/F, IL-23, and tumour necrosis factor (TNF) (all from eBioScience, an Affymetrix Company, San Diego, CA, USA), 2) IL-22 (Abcam, Cambridge, UK), 3) dickkopf-1 (DKK-1) (R&D Systems, Minneapolis, MN, USA), 4) calprotectin (CALPRO AS, Norway), 5) CLDN 3 (My BioSource, Inc., San Diego, CA, USA), and 6) iFABP (Hycult Biotech, Uden, the Netherlands). The above assays were performed according to the manufacturer’s protocols. The ELISA for osteoprotegerin (OPG) was done using recombinant human OPG as a standard and antibodies specific for human OPG (all from R&D Systems). Briefly, mouse monoclonal antibody was used as the capture antibody while goat polyclonal biotinylated antibody was applied as the detection antibody. Streptavidin-peroxidase conjugate (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) followed by o-phenylenediamine dihydrochloride (OPD) (Sigma, ) was added to develop the enzymatic reaction. The concentrations of CALP were determined in faecal samples, CLDN3 in both sera and urine samples, and others in sera samples only.
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