Fetal bovine serum (fbs)

Human esophageal squamous carcinoma TE11 cell line were purchased from RIKEN (Saitama, JAPAN) and were maintained in RPMI medium with high glucose (Life Technologies, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies), 100 mg/mL penicillin G, and 50 μg/mL streptomycin (Life Technologies) at 37°C in a humidified atmosphere containing 5% CO_2. 293T cells were purchased from the Korean Cell Line Bank (Seoul, Korea) and cultured in DMEM with 10% fetal bovine serum (FBS).
The packaging of vector was obtained by transfection of 293T. NF #8 cells were transduced with lentivirus-expressing human FLAG-Twist1 and FLAG control vector and selected with hygromycin (Sigma-Aldrich). CAF#8 cells were infected with a lentivirus encoding a non-specific (NS) shRNA or Twist1 shRNA using Polybrene (Millipore) and selected with 2 ug/ml puromycin as decribed previously [14 (link)].

The SNU484 and SNU638 cell lines were obtained from the Korean Cell Line Bank and they were incubated in RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin in 100-mm dishes under normal conditions at 37 °C with a 5% CO₂-moistened environment. The following antibodies were obtained from the following commercial sources: Antibodies to PARP, caspase-9, caspase-3, Mst1, Mst2, Mob1, p-Mob-1, LATS1, YAP, p-YAP, Sav1, CTGF, MMP9, E-cadherin, Vimentin, and GAPDH were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies to Rassf1 and CTGF were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). UA was obtained from Cayman chemical company (Ann Arbor, Michigan, USA).

Human breast cancer cell lines (MCF-7 and MDA-MB-231) were purchased from the Korean Cell Line Bank (Seoul, South Korea), and they were cultivated in RPMI 1640 media supplemented with 10% fetal bovine serum, 1% penicillin, and streptomycin at 37ºC in a humidified incubator with 5% CO_2. RPMI 1640 medium, fetal bovine serum, phosphate buffered saline (PBS), and penincillin-streptomycin were obtained from Gibco, USA. Recombinant human CXCL10 was purchased from R&D systems (Minneapolis, MN).