Magnesium sulfate heptahydrate (MgSO4·7H2O), sodium chloride (NaCl), and potassium chloride (KCl) were obtained from Chengdu Chron Chemicals Co., Ltd. (Chengdu, China). Copper (II) sulfate pentahydrate (CuSO4·5H2O) was purchased from Chongqing Chuandong Chemical Group Co., Ltd. (Chongqing, China). The Calcium chloride (CaCl2) was the product of Tianjin Damao Chemical Reagent Factory (Tianjin, China). Zinc nitrate hexahydrate (Zn (NO3)2·6H2O, 99%) was purchased from Adamas-beta Co., Ltd. (Shanghai, China). Melamine, DTNB, and zinc acetate dihydrate (C4H6O4Zn∙2H2O) were purchased from Macklin (Shanghai, China). The rabbit plasma (with sodium citrate as the anticoagulant), D (+)-glucose, D (+)-lactose, 2-methylimidazole, vitamin B6 (VB6), vitamin B3 (VB3), vitamin B5 (VB5), ATCh (≥99), and AAO (263 U/mg) were purchased from Shanghai YuanYe Biological Technology Co., Ltd. (Shanghai, China). L (+)-Glutamic acid and AAP were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). L-Lysine was purchased from Chengdu Huaxia Chemical Reagent Co., Ltd. (Chengdu, China). D-phenylalanine was purchased from TargetMol (Boston, MA, USA). Normal human serum was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).
D glucose
Manufactured by Yuanye Bio-Technology
Sourced in China
D-glucose is a monosaccharide, the simplest form of carbohydrate. It is the primary source of energy for many organisms and is commonly used in laboratory settings as a standard reagent.
Automatically generated - may contain errors
Lab products found in correlation
D mannose, by Yuanye Bio-Technology (5 mentions)
D galactose, by Yuanye Bio-Technology (4 mentions)
L arabinose, by Yuanye Bio-Technology (3 mentions)
Magnesium sulfate heptahydrate, by Chron Chemicals (2 mentions)
Rhamnose, by Yuanye Bio-Technology (2 mentions)
D xylose, by Yuanye Bio-Technology (2 mentions)
L rhamnose, by Yuanye Bio-Technology (2 mentions)
D fucose, by Yuanye Bio-Technology (2 mentions)
D arabinose, by Yuanye Bio-Technology (2 mentions)
Chlorogenic acid, by Yuanye Bio-Technology (2 mentions)
Melamine, by Maclin Biochemical Technology (1 mentions)
Zinc acetate dihydrate, by Maclin Biochemical Technology (1 mentions)
Normal human serum, by Solarbio (1 mentions)
Potassium persulfate, by Maclin Biochemical Technology (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
Phenol, by Maclin Biochemical Technology (1 mentions)
Deae sepharose fast flow, by GE Healthcare (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Maclin Biochemical Technology (1 mentions)
Cellulase, by Meilun (1 mentions)
Monosaccharide standard, by Merck Group (1 mentions)
13 protocols using d glucose
1
Aqueous Inorganic Salts and Organic Compounds
2
Cellulase Enzyme Characterization
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Cellulase was supported by Dalian Meilun Biological Technology Co., Ltd (Dalian, China). DEAE Sepharose™ fast flow was purchased from GE Healthcare Life Science (Piscataway, NJ, USA). Phenol, potassium persulfate, 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). Sodium hydroxide, ethanol, chloroform, N-butanol, sodium chloride and sulfuric acid were of analytical grade and were obtained from Guangzhou Reagent Co., Ltd (Guangzhou, China). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) was purchased from Sigma Life Science (St. Louis, MO, USA). Rhamnose, l -arabinose, l -fucose, dl -xylose, d -mannose, d -glucose, and d -galactose were purchased from Shanghai Yuanye Bio-Technology Co. Ltd (Shanghai, China). All other chemicals and reagents used were of analytical grade.
3
Sweet Potato Bioactive Compounds Profiling
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Sweet potatoes (cultivar “Xinxiang”) were purchased from a local market in Hangzhou, Zhejiang Province, China. Taka-diastase, amyloglucosidase, pancreatin, d-glucose, and inulin were purchased from Yuanye Bio-Tech Co., Ltd. (Shanghai, China). de Man, Rogosa and Sharpe (MRS) broth was purchased from Hope Bio-Technology Co., Ltd. (Qingdao, China). Monosaccharide standards (l-arabinose, l-fucose, l-rhamnose, d-galactose, d-glucose, d-mannose, d-ribose, d-glucuronic acid, and d-galacturonic acid) and SCFA standards (acetic acid, propionic acid, and n-butyric acid) were purchased from Sigma-Aldrich Chemical Co. (Shanghai, China). All other chemicals and reagents used in this study were of analytical grade. L. plantarum (RBHZ 68), L. reuteri (MFHZ 18), L. acidophilus (GFHZ 41), and L. rhamnosus (MFHZ 77) were gifted by the Vegetable Processing Laboratory at the Food Science Institute at the Zhejiang Academy of Agricultural Sciences.
4
Osteoblast Precursor Cell Culture
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The MC3T3-E1 murine embryonic osteoblast precursor cell line was obtained from the Chinese Academy of Sciences cell library (Shanghai, China) and cultured in α-MEM medium (8121506, Gibco, Waltham, MA, USA). Cells were seeded into 25-cm2 flasks and treated with or without one or both of 10−3 mmol/L liraglutide and 22.4 mmol/L D-glucose (S11022, Yuanye, Shanghai, China) for 24 h.
5
Isolation and Characterization of Polygonatum sibiricum Polysaccharides
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Polygonatum sibiricum were purchased from a local market (Shenyang, Liaoning, China). HepG2 cell line was purchased from Stem Cell Bank, Chinese Academy of Sciences. DEAE-52 cellulose and Sephadex G-100 were obtained from Solarbio Co., Ltd (Beijing China). Monosaccharide standards (D-glucose, D-mannose, D-galactose, L-arabinose, D-xylose, L-rhamnose, and D-fucose) were purchased from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai China). Fetal bovine serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), trypsin EDTA, penicillin and streptomycin were obtained from Gibco (Grand Island, NY, USA). Caspase-3 and caspase-9 kit were purchased from KeyGEN Biotechnology Co., Ltd. (Jiangsu, China). All other chemicals were of reagent grade.
6
Analytical Characterization of Sarsasapogenin
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d -glucose of analytical grade and sarsasapogenin were purchased from Shanghai Yuanye Biotechnology Co. (Shanghai, China). Other reagents used were from Sinopharm Chemical Reagent Co. (Shanghai, China) and were analytically pure. Distilled water was filtered using a Milli-Q system (Millipore, Bedford, MA, USA). A SpectraMax 190 light absorption microplate reader (Molecular Devices, San Jose, CA, USA) and an Antaris II Fourier transform near infrared spectrometer (Thermo Scientific Inc., Madison, WI, USA) were used for the analyses.
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7
Hawthorn Polyphenol Extraction Protocol
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Hawthorn of the variety “Big Venus” was purchased in local supermarket (Baoding, China) at a fully mature ready-to-eat stage. Polyphenols including epicatechin (≥96%) and chlorogenic acid (98%), monosaccharides standards (D-galacturonic acid (98%), D-glucose (98%), D-mannose (98%), D-rhamnose (98%), D-galactose (98%), D-fucose (98%), D-arabinose (98%)), cellulase (400 U/mg), D3520 macroporous resin, phenol and 3-phenylphenol were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals and reagents were of analytical grade and obtained from Baoding Qianyue Trading Co., Ltd. (Baoding, China).
8
Extraction and Analysis of C. axillaris Fruit
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C. axillaris fruit was purchased from Ganzhou, Jiangxi Province, China. α-amylase, pepsin, trypsin and monosaccharide standards (L-fucose, L-Fuc; L-rhamnose, L-Rha; L-arabinose, L-Ara; D-glucosamine, D-GlcN; D-glactose, D-Gal; D-glucose, D-Glc; L-xylose, L-Xyl; D-mannose, D-Man; L-fructose, L-Fru; D-galacturonic acid, D-GalA; D-guluronic acid, D-GulA) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Pancreatin was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Inulin, resazurin and fatty acid standard (acetic acid, propionic acid, i-butyric acid, n-butyric acid, i-valeric acid, n-valeric acid, n-hexanoic acid) were bought from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Cysteine, heme chloride, pig bile salt and Tween-80 were from Beijing Solarbio Science & Technology Co.,Ltd. (Beijing, China). Dextran standards (Mw 1152, 5000, 11600, 23800, 48600, 80900, 148000, 273000 Da) were bought from Sigma Chemical Co. Ltd. (St. Louis, MO, USA).
9
Comparative Analysis of Oxidative Stress Biomarkers
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Pyridoxine hydrochloride (VB6), D(+)-Lactose, D(+)-Glucose, and superoxide dismutase (SOD) were obtained from Yuanye Biological Technology Co., Ltd. (Shanghai, China). TMB was purchased from Titan Scientific Co., Ltd. (Shanghai, China). Anhydrous ethanol was bought from Chongqing Chuandong Chemical Co., Ltd. (Chongqing, China). H2O2 (30% in water), isopropanol, fructose, sodium chloride, magnesium sulfate heptahydrate, and potassium chloride were obtained from Chengdu Chron Chemicals Co., Ltd. (Chengdu, China). The Oolong tea was purchased from Chongqing Yinrong Trading Co., Ltd. (Chongqing, China). The black tea and green tea were purchased from Unilever Food Co., Ltd. (Shanghai, China). The water used in all experiments was purified by a water purification system (ATSelem 1820A, Antesheng Environmental Protection Equipment Co., Ltd., Chongqing, China).
10
Cholesterol Regulation via Naringin and Rutin
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Naringin and D (+)-glucose were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Rutin was from Shi Feng Biotechnology Co., Ltd. (Shanghai, China). Total cholesterol (TC), triglyceride (TG), and hematoxylin-eosin (H&E) reagent were all purchased from Nanjing Jiancheng Technology Co., Ltd. (Jiangsu, China). CPT1-α, FATP2, SCD-1, ACOX-1, FAS, HRP conjugated goat anti-rabbit IgG (H + L), and GAPDH antibodies were all purchased from Proteintech Group (Chicago, USA). HRP conjugated goat anti-mouse/rabbit IgG (PV-6001) was from Zhongshan Goldenbridge Biotechnology Co., Ltd. (Beijing, China). BCA protein concentration determination kit, RIPA lysate kit, and chemiluminescent assay kit were from Beyotime Biotechnology. (Shanghai, China). All triheptadecenoyl glycerol (T17 : 1 TAG) was purchased from Nu Chek, Inc. (Elysian, USA). All other reagents were at least analytical grade and purchased from Fisher Scientific (Pittsburgh, USA), Sigma-Aldrich Chemical Company (St. Louis, USA), or as specified.
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