Cells and EVs were lysed in RIPA buffer (Merck). Proteins (20 μg for cells and 6 µg for EVs) were electrophoresed on SDS-PAGE. Western blotting analyses were performed using the following antibodies: mouse monoclonal anti-human CD81 (Santa-Cruz Biotechnology; SC-166029), mouse monoclonal anti-human CD63 (Santa-Cruz Biotechnology; SC-5275), rabbit monoclonal anti-human CD9 (abcam; ab92726), rabbit monoclonal anti-human Ang2 (abcam; ab155106), rabbit polyclonal anti-human p-Akt (Cell Signaling technology; #9271), rabbit polyclonal anti-human p-ERK1/2 (Cell Signaling technology; #9101), mouse monoclonal anti-human PI3K (abcam; ab86714), and mouse monoclonal anti-human beta-Actin (Cell Signaling technology; #3700). For the expression of ANG2, p-Akt, p-ERK1/2 and PI3K, the cells were cocultured with 10 µg/mL EVs for 24 h prior to analysis. Densitometry readings were measured using FUSION FX software and were normalized to the corresponding β-actin. The original WB can be found in Figures S1 and S2 .
Mouse monoclonal anti human cd81
Manufactured by Santa Cruz Biotechnology
The Mouse monoclonal anti-human CD81 product is a laboratory reagent used to identify and study the CD81 protein expressed on the surface of various cell types. CD81 is a member of the tetraspanin family of proteins and plays a role in cell signaling, adhesion, and migration. This antibody can be used in techniques such as flow cytometry, immunohistochemistry, and immunoprecipitation to detect and investigate the CD81 protein.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti human cd63, by Santa Cruz Biotechnology (2 mentions)
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Ab155106, by Abcam (1 mentions)
Ab92726, by Abcam (1 mentions)
Ab86714, by Abcam (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Anti gfp, by Thermo Fisher Scientific (1 mentions)
3 protocols using mouse monoclonal anti human cd81
1
Western Blot Analysis of EV and Cell Proteins
2
Exosomal Protein Analysis by Western Blot
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Cells and exosomes pellets were lysed with RIPA lysis buffer (Thermo Scientific, Pierce Protein Biology Products). Proteins (6 μg) were electrophoresed on SDS-PAGE. Western blotting analyses were performed using the following antibodies: mouse monoclonal anti-human CD81 (Santa-Cruz Biotechnology; SC-166029) and mouse monoclonal anti-human CD63 (Santa-Cruz Biotechnology; SC-5275).
3
Antibody Detection in Protein Studies
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A rabbit polyclonal anti-IRSp53 antibody (Merck Millipore), a rabbit polyclonal anti-IRTKS (Bethyl), a mouse monoclonal anti-CA (NIH AIDS Reagent Program), a rabbit polyclonal anti-GFP (Invitrogen), a mouse monoclonal anti-human CD63 (Santa Cruz Biotechnology), a mouse monoclonal anti-human CD81 (Santa Cruz Biotechnology), a rabbit monoclonal anti-human TSG101 (Abcam), and a rabbit polyclonal anti-human ALIX (Covalab) and secondary anti-rabbit Atto647N antibody (Sigma) were used in this study.
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