Vitek 2 ast gn09

The MICs of clinical commonly used antimicrobial agents (listed in Fig. 1) were measured using VITEK 2 AST-GN09 and AST-GN13 test Kit (bioMérieux, Inc.) according to the manufacturer’s instructions. All susceptibility results were interpreted according to the 2017 CLSI performance standards [23 ].Fig. 1

Dendrogram of patterns generated by PFGE of IMP-producing K. pneumoniae isolates. The PFGE types, ST, isolating date of the IMPKpn isolates, IMP variants, the size and type of bla_IMP-carrying plasmids and the MICs of common-used antimicrobial agents were list in this figure. The frame indicated the characterization of IMP-producing K. pneumoniae isolates causing an outbreak. ST sequence type, CAZ ceftazidime, FEP cefepime, TZP piperacillin–tazobactam, IMP imipenem, MEM meropenem, ETP ertapenem, AK amikacin, CIP ciprofloxacin, UT unable type

A string test-positive K. pneumoniae strain (HKE9) was isolated from the blood of an outpatient in the clinical laboratory of the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China. The HKE9 was resistant to most cephems and determined as an ESBL-producing strain. This study was approved by the ethics committee of the First Affiliated Hospital of Xi’an Jiaotong University.
An antibiotic susceptibility test was performed using a VITEK 2 Compact device (bioMérieux Inc., Marcy-l’Étoile, France) with VITEK 2 AST-GN 09. The interpretation of susceptibility data followed the CLSI M100 31st Edition. The HKE9 strain was tested for ESBL production by disk diffusion test, and K. pneumoniae ATCC 700603 (positive) and E. coli ATCC 25922 (negative) were used as quality control and performed as required in the manufacturer’s handbook.