A burn skin wound model was used to measure CD26 expression during wound healing and determine the scar-reducing effects induced by chemically targeting CD26 in vivo. C57/B6 mice (4–6 weeks old) were randomly divided into 3 groups (n=5 per group) as follows: I. Control group, II. Burn wound group, III. Sitagliptin-treated group. I. Control group: Mice were without any treatment. II. Burn wound group: The burn wound group comprised mice with wounds 8 mm in length obtained after the procedure, similar to previous reports [15 (link)]. The procedure was as follows: after anesthesia and back hair removal, a customized iron rod with a diameter of 10 mm was preheated for 10 min in a 100°C water bath. Then, the rod was contacted directly on the back skin for 10 s. As a burn wound group, no treatment was given to the wound after scalding until sacrifice at the prescribed time. III. Sitagliptin-treated group: Sitagliptin (10 mg/kg, S5079, Selleckchem, Houston, USA) was administered subcutaneously around the wound edges after burn injury at 24-h intervals and continued until sacrifice. The first dose was immediately administered postburn, and the final dose was given 1 h before tissue collection. Tissue samples were harvested on day 14 and stored at −80°C until further gene expression quantification and histopathologic analyses were performed.
Sitagliptin
Manufactured by Selleck Chemicals
Sourced in United States
Sitagliptin is a type of pharmaceutical compound used as a key ingredient in the production of certain medications. It functions as a dipeptidyl peptidase-4 (DPP-4) inhibitor, which helps to regulate blood sugar levels in the body. Sitagliptin is a widely used active pharmaceutical ingredient in the development of drugs for the treatment of type 2 diabetes.
Automatically generated - may contain errors
Lab products found in correlation
S5079, by Selleck Chemicals (2 mentions)
Bromodeoxyuridine (brdu), by Abcam (1 mentions)
Dhcr24, by Abcam (1 mentions)
Dpp 4, by Abcam (1 mentions)
Pcmv3 plasmids, by Sino Biological (1 mentions)
Methotrexate mtx, by Selleck Chemicals (1 mentions)
β actin, by Abcam (1 mentions)
Vildagliptin, by LGC (1 mentions)
3h thymidine, by Cytiva (1 mentions)
Rpmi 1640 medium, by GE Healthcare (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Fetal calf serum (fcs), by GE Healthcare (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Hpmecs, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Enos mice, by Jackson ImmunoResearch (1 mentions)
6 protocols using sitagliptin
1
CD26 Modulation in Burn Wound Healing
2
Cell Culture and Transfection Reagents
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Primary antibodies were provided by following sources: DPP4, DHCR24, BrdU, and β-Actin (Abcam, Cambridge, MA, USA); Methotrexate (MTX) and DPP4 inhibitor Sitagliptin were obtained from Selleckchem (Houston, TX, USA). pCMV3 plasmids encoding empty vector (EV) or DCHR24 were obtained from Sinobiological Inc. (Beijing, China). Lentiviral shRNA plasmids encoding scramble control, shDHCR24, and shDPP4 were provided by Genecopoeia Inc. Human GTN cell lines JEG3 JAR, and BEWO were provided by American Type Culture Collection (Manassas, VA, USA). These GTN cell lines were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Hyclone, Logan, UT), 4 mM glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin.
3
Characterization of Monoclonal Antibodies for Flow Cytometry
4
Transfecting miR-23b-3p in HPMECs and 293T Cells
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The HPMEC line was purchased from PromoCell and cultured in PromoCell Cell Growth Medium at 37 °C. The DPP4 inhibitor (sitagliptin) was purchased from Selleck Chemicals. When the cells reached 70% confluence, RNA was transfected into the HPMECs and 293T cells using Lipofectamine®3000 (Invitrogen). Overexpression and inhibition of miR-23b-3p were achieved by
5
Tadpole Feeding Regulation by Inhibitors
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The Dpp4 inhibitor sitagliptin (100 mM, S5079; Selleck, Houston, TX, USA) and/or SGLT inhibitor LX4211 (Sotagliflozin, 100 mM, HY-15516; MedChemExpress, South Brunswick Township, NJ, USA) were administered to the tadpoles 2 h before feeding (Figure 3A). Tadpoles were kept in an inhibitor-free solution during the 2h-feeding to avoid unexpected effects of the inhibitors on the appetite of tadpoles. The treatment and eating cycles were repeated twice daily.
6
Endothelial Nitric Oxide Synthase in Mice
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All animal experiments were performed according to the protocols approved by the Institutional Committee for the Use and Care of Laboratory animals. Endothelial nitric oxide synthase-deficient (eNOS -/-) mice with a C57BL/6 background were obtained from Jackson Laboratories. Wild-type (WT) and eNOS -/-mice (8 weeks of age) were randomly assigned and administered saline or Sitagliptin (15 mg/kg/d, Selleckchem) orally for 12 weeks. Mice were maintained on a 12-hour light-dark cycle. Under anesthesia, the mice were perfused with phosphate-buffered saline, followed by 4% buffered paraformaldehyde. Mice were euthanized, and blood and aortic valve samples were collected
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