smFRET pulse interleaved excitation (PIE) experiments were carried out on Zeiss LSM 710 microscope equipped with PicoQuant LSM upgrade kit for time-resolved measurements. As an excitation source two LHD lasers with emission wavelengths of 485 nm and 640 nm were used. The confocal volume was maintained by using water (C-Apochromat 40x/1.2 NA) objective and focusing the emitted light onto a 35 μm pinhole. Donor and acceptor signals were separated from each other by using a second dichroic mirror (545nm) and filters: 520/35 bandpass and 635 longpass, respectively and further recorded by two avalanche photodiodes. For checks of the 30-nt DNA hairpin conformation and PA-Nter processivity, ~40 μl droplet was placed in home-made chambers consisting of cut 0.5 ml Eppendorf tubes glued (Norland Optical Adhesive 63) to a glass cover slip (#1.5, high precision Carl Roth) sealed with parafilm. The measurement was done for an hour in the same buffer as for FCCS, supplemented with 1mM Trolox. The histogram analysis was done using SymPhoTime ver 5.3.2.3 with an implemented PIE_FRET_2D_burst_analysis_05 script (PicoQuant).
Lsm 710 microscope
Manufactured by PicoQuant
Sourced in Germany
The LSM 710 microscope is a high-performance laser scanning confocal microscope designed for advanced imaging applications. It features a modular design, allowing for customization to meet specific research requirements. The LSM 710 offers precise control over illumination, detection, and optical sectioning, enabling users to capture detailed, high-resolution images of biological samples.
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Lab products found in correlation
2 protocols using lsm 710 microscope
1
smFRET Experiments on Zeiss LSM 710
2
Fluorescence Lifetime Measurements of Quantum Dots
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Fluorescence lifetime
measurements were performed using the Zeiss LSM710 microscope with
an LSM upgrade kit (PicoQuant, Berlin, Germany), as previously described,68 (link) with the detection range optimized for currently
used QDs.
measurements were performed using the Zeiss LSM710 microscope with
an LSM upgrade kit (PicoQuant, Berlin, Germany), as previously described,68 (link) with the detection range optimized for currently
used QDs.
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