For promoter activity assays in E. coli LMG194, cells were inoculated at an OD600 of 0.05 and aerobically grown at 37 °C in microtiter plates within a Tecan Infinite F500 system (Tecan). OD600 and luminescence were measured every 10 minutes for 8 h. Arabinose was added at a final concentration of 0.0001% (w/v) after the cells reached an OD600 of 0.2. Data are reported as relative light units (RLU) in counts per second per milliliter per OD600.
For reporter activity assays with P. luminescens, the OD600, which initially was adjusted to 0.05, the cells were aerobically grown at 30 °C in microtiter plates with a Tecan Infinite F500 system (Tecan). The OD and fluorescence intensity of mCherry (560 nm excitation, 612 nm emission, 20 nm bandwidth) were measured after 8, 24 and 28 h of incubation. Data are reported as relative fluorescence units (RFU). Raw fluorescence data were normalized with the optical density (OD600) of the respective culture.
For reporter activity assays with P. luminescens, the OD600, which initially was adjusted to 0.05, the cells were aerobically grown at 30 °C in microtiter plates with a Tecan Infinite F500 system (Tecan). The OD and fluorescence intensity of mCherry (560 nm excitation, 612 nm emission, 20 nm bandwidth) were measured after 8, 24 and 28 h of incubation. Data are reported as relative fluorescence units (RFU). Raw fluorescence data were normalized with the optical density (OD600) of the respective culture.