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2 protocols using fitc conjugated anti mouse cd49b

1

Multiparametric Flow Cytometry Analysis

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The following monoclonal antibodies were used during the assay: Brilliant violet (BV)510-conjugated anti-mouse CD3 (BD-Biosciences), BV510-conjugated anti-mouse γ/δTCR (BD-Biosciences) recognizing delta chain, fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 (BD-Biosciences), FITC-conjugated anti-mouse CD107a (BD-Biosciences), FITC-conjugated anti-mouse CD49b (BD-Biosciences), phycoerythrin (PE)-conjugated anti-mouse TIM-3 (R&D Systems), PE-conjugated anti-mouse Gal-9 (Biolegend), PE-conjugated anti-mouse CD49b (BD-Biosciences), peridinin chlorophyll a protein (PerCP)-conjugated anti-mouse CD45 (Exbio), PE-Cy7-conjugated anti-mouse CD25 (BD-Biosciences), allophycocyanin (APC)-conjugated anti-mouse TIM-3 (R&D Systems), APC-conjugated anti-mouse FoxP3 (eBioscience) and APC-H7-conjugated anti-mouse CD8 (BD-Biosciences). Control antibodies included isotype-matched rat and hamster antibodies.
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2

Comprehensive Immune Cell Profiling Panel

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The following monoclonal antibodies were implemented: Brilliant Violet (BV) 421-conjugated anti-mouse PD-1 (BD Pharmingen), BV510-conjugated anti-mouse CD3 (BD Pharmingen), BV510-conjugated anti-mouse g/d (BD Pharmingen), fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 (BD Pharmingen), FITC-conjugated anti-mouse CD8 (BD Pharmingen), FITCconjugated anti-mouse CD107a (BD Pharmingen), FITCconjugated anti-mouse CD49b (BD Pharmingen), FITC-conjugated anti-mouse g/d (BD Pharmingen), phycoerythrin (PE)-conjugated anti-mouse g/d (BD-Pharmingen), PE-conjugated anti-mouse TIM-3 (R and D Systems), PE-conjugated anti-mouse Gal-9 (Biolegend), PE-conjugated anti-mouse CD49b (BD Pharmingen), PE-conjugated anti-mouse CD8 (BD Pharmingen), peridinin chlorophyll a protein (PerCP)-conjugated anti-mouse CD3 (BD Pharmingen), PerCPconjugated anti-mouse CD45 (BD Pharmingen), PE-Cy7conjugated anti-mouse CD25 (BD Pharmingen), allophycocyanin (APC)-conjugated anti-human TIM-3 (R and D Systems), APCconjugated anti-human FoxP3 (eBioscience) and APC-H7conjugated anti-human CD8 (BD Pharmingen). Control antibodies included isotype-matched FITC-conjugated, PE-conjugated, PerCPconjugated and APC-conjugated rat antibodies.
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