Recombinant mouse scil 12

Manufactured by Miltenyi Biotec

Recombinant mouse scIL-12 is a laboratory reagent produced by Miltenyi Biotec. It is a recombinant form of the mouse interleukin-12 cytokine. Interleukin-12 is a protein involved in the regulation of immune responses.

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Lab products found in correlation

Polyacrylamide gel, by Bio-Rad (2 mentions) Recombinant human il 15, by BioLegend (2 mentions) Dbco nhs ester, by Vector Laboratories (2 mentions) Sulfo cyanine7 nhs ester, by Lumiprobe (2 mentions) Recombinant human il 2, by Thermo Fisher Scientific (2 mentions) Ifnγ elisa, by R&D Systems (1 mentions) Ifn γ elisa, by BioLegend (1 mentions)

2 protocols using recombinant mouse scil 12

Cytokine-Conjugated Polymer Nanomedicines

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Unless otherwise specified, reagents were used as received without further purification. Recombinant human IL-2 (200-02, Peprotech), recombinant human IL-15 (570,308, Biolegend), and recombinant mouse scIL-12 (130-096, Miltenyi Biotech) were sourced as indicated. Sulfo-Cyanine7 NHS ester (Lumiprobe), DBCO-NHS ester (1160, Click Chemistry Tools), poly(ethylene glycol) methyl ether azide (20 kDa, Nanocs), polyacrylamide gels (Bio-Rad, 4–15 wt%), and IFNγ ELISA (DY485, R&D Systems) were sourced as indicated.

Perdue L.A., Do P., David C., Chyong A., Kellner A.V., Ruggieri A., Kim H.R., Salaita K., Lesinski G.B., Porter C.C, & Dreaden E.C. (2020). Optical Control of Cytokine Signaling via Bioinspired, Polymer-Induced Latency. Biomacromolecules, 21(7), 2635-2644.

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Cytokine-mediated T cell activation

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Unless otherwise specified,
reagents were used as received without further purification. Recombinant
human IL-2 (200-02, Peprotech), recombinant human IL-15 (570308, Biolegend),
recombinant mouse scIL-12 (130-096, Miltenyi Biotec), and recombinant
human IL-12 (CT050-HNAH, Sino Biological). Sulfo-Cyanine7 NHS ester
(Lumiprobe), DBCO-NHS ester (1160, Click Chemistry Tools), NHS-PEG-TCO
ester (A137-10, Click Chemistry Tools), poly(ethylene glycol)methyl
ether DBCO (20 kDa, A120-100, Click Chemistry Tools), and poly(ethylene
glycol)methyl ether azide (20 kDa, Nanocs). Polyacrylamide gels (Bio-Rad,
4–16 wt %). IFNγ ELISA (430104, Biolegend). CD19 antibody
(BD Biosciences Clone SJ25C1) and PD-L1 antibody (eBioscience Clone
MIH1).

Birnbaum L.A., Sullivan E.C., Do P., Uricoli B., Raikar S.S., Porter C.C., Henry C.J, & Dreaden E.C. (2023). Multicolor Light-Induced Immune Activation via Polymer Photocaged Cytokines. Biomacromolecules, 24(3), 1164-1172.

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