Aav cre gfp

For the deletion of the Bdnf gene selectively in the dentate gyrus, anesthetized adult Bdnf^flox/flox mice underwent bilateral stereotaxic injections of AAV-Cre-GFP or AAV-GFP (0.5 μl /side; Vector Biolabs, Malvern, PA) into the dentate gyrus (coordinates: AP = −2.1 mm, ML = ±1.5 mm, DV = −2.3 mm from Bregma), at a rate of 0.1 μL/min with a 33-gauge stainless steel injector connected to a UMP3 micro syringe pump (World Precision Instruments, Sarasota, FL). Additional 5 min were allowed for diffusion and prevention of backflow. Behavioral experiments were conducted 21 days after AAV injection. For intra-dentate gyrus microinjection of inhibitors of AKT and p300 HAT, the mice were anesthetized with 4% chloral hydrate (400 mg/kg, i.p.). AKTi and C646 (0.5 μl/side) were injected bilaterally into the dentate gyrus of adult C57BL/6J mice at a rate of 0.5 μl/min with a 33-gauge stainless steel injector. Additional 5 min were allowed for diffusion and prevention of backflow, and 30 min later, mice were injected with leptin (5 mg/kg, i.p.).