We obtained 20 GC and 20 CRC tissues from the tissue bank of Nanjing Drum Tower Hospital for RNA‐sequencing analysis. In addition, we collected 20 EBV+ and 20 EBV− GC tissues from Nanjing Drum Tower Hospital. The CRC tissue microarray including 36 paired tumor and normal tissues was obtained from Fudan University Shanghai Cancer Center. Another GC tissue microarray (HStmA150CS02) that contained 74 paired available tumor and normal tissues was obtained from Shanghai Outdo Biotech Company (Shanghai, China).
For clinical validation, we retrospectively collected tumor tissues from 13 patients with GI cancer and an additional 1 patient with melanoma and 1 patient with chest wall sarcoma between September 2018 and March 2021, of whom 6 samples were from patients in a clinical trial (ID: 2016L01455), and the other 9 tissues were from patients who received pembrolizumab in our cancer centre.
For clinical validation, we retrospectively collected tumor tissues from 13 patients with GI cancer and an additional 1 patient with melanoma and 1 patient with chest wall sarcoma between September 2018 and March 2021, of whom 6 samples were from patients in a clinical trial (ID: 2016L01455), and the other 9 tissues were from patients who received pembrolizumab in our cancer centre.