Horse anti rabbit igg ba 1100

Four-µm sections of paraffin slides obtained from patients with PDAC were provided from the pathology department. Primary antibodies—anti-CD3 (ab5690), anti-CD8 (ab17147), anti-CD20 (ab78237), anti-CD66b (ab197678), and anti-α-sma (ab5694)—were purchased from Abcam PLC (Cambridge, UK). A Masson-Goldner staining kit (100485) for collagen staining was bought from Merck KGaA (Darmstadt, Germany). Secondary antibodies—horse anti-mouse IgG (BA-2000) and horse anti-rabbit IgG (BA-1100)—were purchased from VECTOR Laboratories (Burlingame, CA, USA), along with the Avadin/Biotin Blocking Kit (SP-2001), the alkaline phosphatase enzyme detection system (ABC-AP) (AK-5000), and the ImmPACT Vector Red Alkaline Phosphatase Substrate Kit (SK-5105).

Primary antibodies, anti-CD3 (ab5690), anti-CD8 (ab17147), anti-CD20 (ab78237), and anti-CD66b (ab197678) were purchased from Abcam Plc. (Cambridge, Cambs, UK). Secondary antibodies, Horse Anti-Rabbit IgG (BA-1100), and Horse Anti-Mouse IgG (BA-2000) were purchased from VECTOR Laboratories (Burlingame, CA, USA). Isotype controls against IgG were used as appropriate.
Avidin/Biotin Blocking Kit (SP-2001), ABC-AP (Alkaline phosphatase enzyme detection system, AK-5000), and ImmPACT Red Alkaline Phosphatase Substrate Kit (SK-5105) were purchased from VECTOR Laboratories (Burlingame, CA, USA).