Elute uplc system

Bruker Daltonics (Bremen, Germany) Impact II ESI-Q-TOF System equipped with Bruker Daltonics Elute UPLC system (Bremen, Germany) was used for screenings of the compounds. The standards for identifying the m/z with high-resolution Bruker TOF MS and the exact retention times of each analyte were used as obtained during the chromatographic analysis. This instrument was operated using the Ion Source Apollo II ion funnel electrospray source. The capillary voltage was 2500 V, the nebulizer gas was 2.0 bar, and the dry gas (nitrogen) flow rate was 8 L/min with the dry temperature at 200 °C. The mass accuracy was <1 ppm; the mass resolution was 50,000 FSR (full sensitivity resolution), and the TOF repetition rate was up to 20 kHz.

An amount of 10 mg of each dried plant extract was weighed and then solubilized in 100 µL DMSO (dimethyl sulfoxide). The extracts were diluted up with methanol: water (80:10 %), vortexed and centrifuged at 3000 rpm for 10 min. Finally, the samples were injected into the system. All solvents used were of HPLC grade.
The test samples were analyzed by the Bruker impact II ESI-Q-TOF system, provided with Bruker Dalotonik Elute UPLC system and characterized by mass accuracy of less than one ppm; the mass resolution was 50,000 FSR (Full Sensitivity Resolution) and the TOF repetition rate was up to 20 kHz.
Chromatographic separation was run through the Bruker Solo C 18-2 column with a size of 100 mm × 2.1 mm and with a particle size of 2 μm. The mobile phase comprises water, formic acid (HCOOH, 0.05%) and acetonitrile. The linear gradient started with water–formic acid (5–80%) from 0 to 27 min. Then, 95% acetonitrile (27–29 min) and 5% acetonitrile (29.1 min). The analysis was carried out using MetaboScape version 5.

A Bruker Daltonik Impact II ESI-Q-TOF System with a Bruker Daltonik Elute UPLC system (Bremen, Germany) was used to screen the compounds of interest in the extracts. The extract samples were dissolved in 2 ml DMSO and made up to 50 ml using acetonitrile. Then, the samples were centrifuged at 4,000 rpm for 2 min and transferred to autosampler where 3 μL was injected (59 standards were used to identify ms/z and the retention time).
After chromatographic separation, high-resolution Bruker TOF MS was utilized to determine m/z and the exact retention duration of each analyte. The Ion Source Apollo II ion Funnel electrospray source was used to power this instrument. The capillary voltage was 2,500 V, the nebulizer gas pressure was 2 bar, the dry gas (nitrogen) flow rate was 8 L/min, and the dry temperature was 200°C. The mass resolution was 50,000 FSR, and the mass accuracy was one ppm (Full Sensitivity Resolution). The repetition rate of the TOF was up to 20 kHz. Chromatographic separation was achieved using an Elute UHPLC connected to a Bruker Impact II QTOFMS.