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Murine 4t1 breast cancer cells

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The Murine 4T1 breast cancer cells are a well-established cell line derived from a spontaneously occurring mammary tumor in a BALB/c mouse. These cells are widely used in cancer research to study various aspects of breast cancer biology and for the development of novel therapeutic approaches.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) U87 luciferase cells, by PerkinElmer (2 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) B16 melanoma cells, by American Type Culture Collection (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Fetal bovine serum (fbs), by GeneDireX (1 mentions) Fetal bovine serum (fbs), by Caisson (1 mentions) Aspirin, by Merck Group (1 mentions) Penicillin streptomycin amphotericin b, by Caisson (1 mentions) Dulbecco modified eagle medium (dmem), by Caisson (1 mentions)

Spelling variants of this product

4T1 cells (122 citations) 4T1 murine breast cancer cells (21 citations) 4T1 breast cancer cells (12 citations) Murine 4T1 (10 citations) 4T1 murine breast cancer (6 citations) Murine 4T1 cells (5 citations) 4T1 breast cancer (4 citations)

6 protocols using murine 4t1 breast cancer cells

1

Culturing Melanoma and Breast Cancer Cells

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B16, melanoma cells (ATCC, Manassas, VA) were cultured in T-175 flasks using DMEM (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS), 1% Pen Strep (Penicillin Streptomycin, Gibco, Life Technologies, Grand Island, NY). Murine 4T1 breast cancer cells (ATCC, Manassas, VA) were cultured in T-175 flasks in RPMI 1640 (Gibco, Life Technologies, Grand Island, NY) with the same supplements. The cells were maintained at 37°C, 5% CO2, and 95% relative humidity. The medium was changed every other day and the cells were passaged at 75-90% confluency using TyrpLE Express Enzyme (Gibco, Live Technologies, Grand Island, NY).
Aydin O., Lorsung R., Chandran P., Cohen G., Burks S.R, & Frank J.A. (2019). The proteomic effects of pulsed focused ultrasound on tumor microenvironments of murine melanoma and breast cancer models. Ultrasound in medicine & biology, 45(12), 3232-3245.
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2

Authenticated Cell Lines for Cancer Research

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Human MDA-MB-231 (MDA-231) and MDA-MB-436 (MDA-436) breast cancer cells, human H1703 lung cancer cells and murine 4T-1 breast cancer cells were purchased from ATCC. The human PC3-ML prostate cancer cell line was derived from the parental PC3 cells as previously described 113 (link). These cell lines were cultured in DMEM (Gibco) - except the 4T-1 cells that were cultured in RPMI (Gibco) - supplemented with 10% fetal bovine serum (FBS, HyClone) and 0.1% gentamicin (Gibco). The human WM793 melanoma cell line and the 1205Lu cell line, derived from a lung metastasis induced in immunocompromised mice grafted with WM793 cells, were a gift from Dr. Edward Hartsough (Pharmacology and Physiology, Drexel University). Cell line authentication was performed by IDEXX BioResearch using single tandem repeat and conducted to determine the species of origin and rule out interspecies contamination performing the CellCheck 9 Plus test. All cell lines were also tested for Mycoplasma contamination by IDEXX on a regular basis using PCR detection and only negative cells were used for this study. See further details in Supplementary Methods.
DiNatale A., Kaur R., Qian C., Zhang J., Marchioli M., Ipe D., Castelli M., McNair C.M., Kumar G., Meucci O, & Fatatis A. (2022). Subsets of cancer cells expressing CX3CR1 are endowed with metastasis-initiating properties and resistance to chemotherapy. Oncogene, 41(9), 1337-1351.
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3

Murine 3T3-L1, RAW264.7 and 4T1 Cell Culture

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Mouse 3T3-L1 fibroblast and RAW264.7 macrophage were kindly provided by Dr. Lin of the National Taiwan University and Dr. Tsai of the National Taiwan Normal University (Taipei, Taiwan), respectively. The original cell lines were purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institute (Hsinchu, Taiwan). Mouse 3T3-L1 and RAW264.7 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Caisson, Smithfield, UT, USA) containing 10% heat-inactivated calf serum (BS, Gibco, Grand Island, NY, USA) and fetal bovine serum (FBS, Genedirex, Las Vegas, NV, USA), respectively. Murine 4T1 breast cancer cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in DMEM containing 10% heat-inactivated FBS with 1% penicillin/streptomycin/amphotericin B (Caisson) at 37°C in an incubator containing a humidified atmosphere of 5% CO2. Aspirin was purchased from Sigma (St. Louis, MO, USA), dissolved in dimethyl sulfoxide (DMSO, Sigma) as a stock solution, and then stored at −20°C. The concentration of DMSO in the vehicle group was equal to the 0.25% DMSO in the highest (5 mM) dose of Aspirin.
Hsieh C.C, & Huang Y.S. (2016). Aspirin Breaks the Crosstalk between 3T3-L1 Adipocytes and 4T1 Breast Cancer Cells by Regulating Cytokine Production. PLoS ONE, 11(1), e0147161.
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4

4T1 Breast Cancer Cell Culture

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Murine 4T1 breast cancer cells were purchased from American Type Culture Collection (ATCC). The cells were regularly checked for mycoplasma contamination. The 4T1 cancer cells were cultured in Dulbecco's Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C in a humidified environment containing 5% CO2, respectively. Before experiments, the cells were pre-cultured until confluence was reached.
Zhao Z., Chen C., Wu W., Wang F., Du L., Zhang X., Xiong Y., He X., Cai Y., Kwok R.T., Lam J.W., Gao X., Sun P., Phillips D.L., Ding D, & Tang B.Z. (2019). Highly efficient photothermal nanoagent achieved by harvesting energy via excited-state intramolecular motion within nanoparticles. Nature Communications, 10, 768.
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5

Murine Breast and Glioma Cell Culture

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Murine breast cancer cells (4T1) were obtained from American Type Culture Collection (ATCC) and human glioma cancer U87-luciferase cells were obtained from PerkinElmer. Those cells were cultured in normal RPMI-1640 or DMEM culture medium with 4.5 g / L of D-glucose (containing 10 % of fetal bovine serum and 1% of penicillin/streptomycin) at 37 °C under 5 % CO2.
Song G., Kenney M., Chen Y.S., Zheng X., Deng Y., Chen Z., Wang S.X., Gambhir S.S., Dai H, & Rao J. (2020). Carbon-coated FeCo nanoparticles as sensitive magnetic-particle-imaging tracers with photothermal and magnetothermal properties. Nature biomedical engineering, 4(3), 325-334.
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6

Murine Breast and Glioma Cell Culture

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Murine breast cancer cells (4T1) were obtained from American Type Culture Collection (ATCC) and human glioma cancer U87-luciferase cells were obtained from PerkinElmer. Those cells were cultured in normal RPMI-1640 or DMEM culture medium with 4.5 g / L of D-glucose (containing 10 % of fetal bovine serum and 1% of penicillin/streptomycin) at 37 °C under 5 % CO2.
Song G., Kenney M., Chen Y.S., Zheng X., Deng Y., Chen Z., Wang S.X., Gambhir S.S., Dai H, & Rao J. (2020). Carbon-coated FeCo nanoparticles as sensitive magnetic-particle-imaging tracers with photothermal and magnetothermal properties. Nature biomedical engineering, 4(3), 325-334.
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