96-well Costar ELISA plates (Thermo Fisher Scientific) were coated with CMP-001 at a concentration of 10 μg/mL in PBS. Following coating, the plates were washed with PBS-Tween 20 0.05% (PBS-T), blocked with 5% dry milk in PBS-T, washed again and then incubated with titrated dilutions of murine or human serum. After washing, HRP-conjugated goat anti-mouse Ig (Southern Biotech) or goat anti-human Ig (Southern Biotech) detection antibodies diluted in PBS-T were added. The plate was developed with the addition of TMB substrate (Sigma-Aldrich), followed by 2N H2SO4 stop solution. Color development was read on a plate reader at an absorbance of 450nm. Murine serum samples were obtained from mice that had previously been injected with CMP-001, or from control naïve mice. Human serum samples were obtained from clinical trial patients pre- and post-treatment with CMP-001.
Hrp conjugated goat anti mouse ig
Manufactured by Southern Biotech
HRP-conjugated goat anti-mouse Ig is a secondary antibody that binds to mouse immunoglobulins. It is conjugated to horseradish peroxidase (HRP), an enzyme used in various detection methods.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hrp conjugated goat anti mouse ig
1
CMP-001 ELISA Antibody Quantification
2
Immunolocalization of TsCstN in T. spiralis-infected Muscle
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T. spiralis-infected mice muscles were obtained and immediately fixed in fixing solution (4% paraformaldehyde, 1 × PBS, pH 7.4) before the preparation of paraffin-embedded sections and immunolocalization as previously described with some modifications [34 (link)]. In summary, 3-μM-thick sections were deparaffinized, rehydrated, retrieved antigenic epitopes and inactivated endogenous peroxidase before blocking with 3% FBS in 1 × PBS at RT for 1 h. Subsequently, the sections were incubated overnight with the 1:100 mouse anti-TsCstN serum or 1:100 pre-immunized serum at 4°C. After washing thrice with PBST, the sections were overlaid with 1:1,000 HRP-conjugated goat anti-mouse Ig (Southern Biotech) and incubated at RT, for 1 h. The chromogenic result was developed using AEC staining kit (Sigma-Aldrich) according to the manufacturer’s instructions. The slides were examined using a light microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!