Western blot assays were conducted as previously described [13 (link)]. Briefly, equal amounts of proteins were separated on 10%–12% SDS-PAGE, transferred onto PVDF membranes (0.45 μM), and blocked with 5% skim milk. Then, the membranes were incubated with various primary antibodies at 4 °C for more than 10 h. Afterwards, the membranes were blotted with HRP-conjugated secondary antibodies. The signal was visualized with ECL (Beyotime Biotechnology, Jiangsu, China) using the LiCor C-DiGit Blot scanner with the LiCor Biosciences Image Studio software. The anti-E cadherin antibody (HECD-1) (1:1000), anti-vimentin antibody (RV202) (1:1000), and anti-TYRO3 antibody (EPR4308) (1:1000) were obtained from Abcam.
Anti e cadherin antibody hecd 1
Manufactured by Abcam
Anti-E cadherin antibody (HECD-1) is a primary antibody that binds to the E-cadherin protein. E-cadherin is a cell-cell adhesion molecule that plays a crucial role in maintaining cell-cell contacts and the integrity of epithelial tissues. This antibody can be used in various research applications, such as immunohistochemistry and Western blotting, to detect and study the expression and localization of E-cadherin in biological samples.
Automatically generated - may contain errors
3 protocols using anti e cadherin antibody hecd 1
1
Western Blot Analysis of EMT Markers
2
Western Blot Analysis for Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blot assays were conducted as previously described [13] . Briefly, equal amounts of proteins were separated on 10%-12% SDS-PAGE, transferred onto PVDF membranes (0.45 μM), and blocked with 5% skim milk. Then, the membranes were incubated with various primary antibodies at 4 °C for more than 10 h. Afterwards, the membranes were blotted with HRP-conjugated secondary antibodies. The signal was visualized with ECL (Beyotime Biotechnology, Jiangsu, China) using the LiCor C-DiGit Blot scanner with the LiCor Biosciences Image Studio software. The anti-E cadherin antibody (HECD-1) (1:1000), antivimentin antibody (RV202) (1:1000), and anti-TYRO3 antibody (EPR4308) (1:1000) were obtained from Abcam.
3
Western Blot Analysis of EMT Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot assays were conducted as previously described [13] . Briefly, equal amounts of proteins were separated on 10%-12% SDS-PAGE, transferred onto PVDF membranes (0.45 μM), and blocked with 5% skim milk. Then, the membranes were incubated with various primary antibodies at 4 °C for more than 10 h. Afterwards, the membranes were blotted with HRP-conjugated secondary antibodies. The signal was visualized with ECL (Beyotime Biotechnology, Jiangsu, China) using the LiCor C-DiGit Blot scanner with the LiCor Biosciences Image Studio software. The anti-E cadherin antibody (HECD-1) (1:1000), anti-vimentin antibody (RV202) (1:1000), and anti-TYRO3 antibody (EPR4308) (1:1000) were obtained from Abcam.
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