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Akt sirna

Manufactured by Qiagen
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Sourced in Germany

The AKT siRNA is a laboratory product designed for research purposes. It is a small interfering RNA (siRNA) molecule that targets the AKT gene, which is involved in various cellular processes. The core function of the AKT siRNA is to temporarily reduce the expression of the AKT gene in cell cultures or other research models, allowing researchers to study the effects of AKT downregulation. This product is intended for use in controlled laboratory settings by trained personnel.

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Lab products found in correlation

Control sirna, by Qiagen (1 mentions) 4d nucleofector system, by Lonza (1 mentions) Jetprime reagent, by Polyplus Transfection (1 mentions)

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SiRNAs (104 citations)

2 protocols using akt sirna

1

AKT Knockdown in RS4;11 Cells

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RS4;11 cells (1 × 106) were transfected with AKT siRNA (Cat no: SI02758406, Qiagen, Germany) and Control siRNA (Cat no: 1027281, Qiagen) using 4D-Nucleofector™ System (Lonza). After 48 h of incubation, cells were lysed and immunoblotted with anti-AKT and other antibodies.
Kuttikrishnan S., Siveen K.S., Prabhu K.S., Khan A.Q., Ahmed E.I., Akhtar S., Ali T.A., Merhi M., Dermime S., Steinhoff M, & Uddin S. (2019). Curcumin Induces Apoptotic Cell Death via Inhibition of PI3-Kinase/AKT Pathway in B-Precursor Acute Lymphoblastic Leukemia. Frontiers in Oncology, 9, 484.
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2

Phb1-KO Hepatocytes Transfection

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Primary Phb1-KO hepatocytes were transfected with 100 nM Nedd8, LKB1 and Akt siRNA (Qiagen) using jetPRIMETM reagent (Polyplus). Controls were transfected with an unrelated siRNA (Qiagen). Protein knockdown was confirmed by Western blotting. siRNA sequences are shown in Supplementary Table V.
Barbier-Torres L., Delgado T.C., García-Rodríguez J.L., Zubiete-Franco I., Fernández-Ramos D., Buqué X., Cano A., Juan V.G., Fernández-Domínguez I., Lopitz-Otsoa F., Fernández-Tussy P., Boix L., Bruix J., Villa E., Castro A., Lu S.C., Aspichueta P., Xirodimas D., Varela-Rey M., Mato J.M., Beraza N, & Martínez-Chantar M.L. (2014). Stabilization of LKB1 and Akt by neddylation regulates energy metabolism in liver cancer. Oncotarget, 6(4), 2509-2523.
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