Uas sec8

Manufactured by Bloomington Drosophila Stock Center

Sourced in United States

The UAS-Sec8 is a genetic tool used in Drosophila (fruit fly) research. It allows for the targeted expression of the Sec8 gene, which is involved in the regulation of vesicle trafficking and exocytosis. The UAS-Sec8 construct can be used in combination with the GAL4 system to spatially and temporally control the expression of Sec8 in Drosophila. This tool can be utilized by researchers studying various cellular and developmental processes in the fruit fly model organism.

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2 protocols using uas sec8

Drosophila Endocytic Trafficking Regulators

Cited 2 times

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All crosses and staging were performed at 25°C. Stocks are described in FlyBase (http://flybase.bio.indiana.edu). UAS-YFP-Rab11, UAS-YFP-Rab11^Q70L, UAS-YFP-Rab11^S25N, UAS-Rab11-RNAi, UAS-Cdc42^V12, UAS-YFP-Rab8^Q67L, UAS-YFP-Rab4, UAS-YFP-Rab14^Q97L, UAS-aPKC-RNAi, UAS-Rho1, Dia⁵ FRT40A, UAS-YFP-Rab5, GMR-gal4, Nuf^EY11342, Rho1^72F, Cdc42⁴ FRT19A, UAS-Sec8, Rab11^EP3017, UAS-Rok-CAT, UAS-LIMK-HA, UAS-MBS-RNAi, and Sqh^AX3; RLC-GFP were provided by the Bloomington Stock Center (Indiana University, Bloomington, IN); UAS-Rab5-RNAi was provided by the Vienna Drosophila RNAi Center (Vienna, Austria); UAS-Rok-RNAi and UAS-PKN-RNAi were provided by the National Institute of Genetics (Mishima, Japan); Shg^R69 FRT42D was provided by C. Micchelli (Washington University, St. Louis, MO); Par6^Δ226 FRT19A and UAS-aPKC^CAAX were provided by C. Doe (University of Oregon, Eugene, OR); Sec5^E10 FRT40A and UAS-Sec5 were provided by T. L. Schwarz (Harvard Medical School, Boston, MA); dRip11^d00190 was provided by the Exelixis Collection (Harvard Medical School); and UAS-Rho1 RNAi, UAS-Cdc42 RNAi, and UAS-Cdc42 were generated by our lab (Warner and Longmore, 2009a (link), b (link)).

Yashiro H., Loza A.J., Skeath J.B, & Longmore G.D. (2014). Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II. Molecular Biology of the Cell, 25(19), 2956-2969.

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Drosophila Genotyping and Maintenance

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All fly stocks were maintained in our laboratory on a standard cornmeal medium at 25 °C. The Actin-GAL4 driver (stock number 4414) and UAS-Sec8 (stock number 9556) were from Bloomington Stock Center (Bloomington, IN, USA). UAS-LRRK2 WT and UAS-LRRK2-R1441C were a generous gift from Prof. Cheng-Ting Chien (National Taiwan University Hospital Yun-Lin Branch, Taipei, Taiwan) [51 (link)].

Ciampelli C., Galleri G., Puggioni S., Fais M., Iannotta L., Galioto M., Becciu M., Greggio E., Bernardoni R., Crosio C, & Iaccarino C. (2023). Inhibition of the Exocyst Complex Attenuates the LRRK2 Pathological Effects. International Journal of Molecular Sciences, 24(16), 12656.

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