Quantstudio pcr systems

Manufactured by Thermo Fisher Scientific

The QuantStudio PCR Systems are a family of real-time polymerase chain reaction (qPCR) instruments designed for gene expression analysis, genotyping, copy number variation, and other applications. The systems feature durable, high-performance optics and thermal cycling technologies to enable accurate and reproducible results.

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Lab products found in correlation

Miscript rt and pcr system, by Qiagen (2 mentions) Mirneasy kit, by Qiagen (2 mentions) Nanodrop nd 1000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

QuantStudio 5 real-time RT-PCR systems QuantStudio 3 and 5 PCR Real-Time Systems StepOne/QuantStudio 6 Flex Real Time PCR Systems StepOne and QuantStudio 5 Real-Time PCR systems QuantStudio® 3 RCR Real-Time PCR systems QuantStudio 7 Pro Real-Time PCR Systems Software QuantStudio™ 7 Flex Real-Time PCR System and Software (v 1.0) Applied Biosystems QuantStudio 3D Digital PCR System QuantStudio™ 3 FlexReal-Time PCR System QuantStudio 12K Flex Rea l-Time PCR System instrument

2 protocols using quantstudio pcr systems

RNA Isolation and qPCR Analysis

Cited 1 time

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RNA was isolated using the RNeasy or miRNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations. The concentration of total RNA was determined by NanoDrop ND-1000.
cDNA for mRNA analysis was prepared using the RevertAidTM H minus First-strand Kit (Thermo Fischer Scientific, Massachusetts, USA). Primers and probes are listed in Additional file 2: Supplementary Table 3. For quantification of miRNAs, miScript RT and PCR system (Qiagen, Hilden, Germany) was used. Raw data analysis was performed by using QuantStudio PCR Systems (Applied Biosystems). Data acquisition and raw data analysis were performed using QuantStudio PCR Systems (Applied Biosystems) with the ΔΔCt method [24 (link)].

Li X., Michels B.E., Tosun O.E., Jung J., Kappes J., Ibing S., Nataraj N.B., Sahay S., Schneider M., Wörner A., Becki C., Ishaque N., Feuerbach L., Heßling B., Helm D., Will R., Yarden Y., Müller-Decker K., Wiemann S, & Körner C. (2022). 5’isomiR-183-5p|+2 elicits tumor suppressor activity in a negative feedback loop with E2F1. Journal of Experimental & Clinical Cancer Research : CR, 41, 190.

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RNA Isolation and qRT-PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was isolated using the RNeasy or miRNeasy Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations. The concentration of total RNA was determined by NanoDrop ND-1000.
cDNA for mRNA analysis was prepared using the RevertAidTM H minus First-strand Kit (Thermo Fischer Scienti c, Massachusetts, USA). Primers and probes are listed in Additional le 2: Supplementary Table 3. For quanti cation of miRNAs, miScript RT and PCR system (Qiagen, Hilden, Germany) was used. Raw data analysis was performed by using QuantStudio PCR Systems (Applied Biosystems). Data acquisition and raw data analysis were performed using QuantStudio PCR Systems (Applied Biosystems) with the ΔΔCt method [23] .

Li X., Tosun O.E., Jung J., Kappes J., Ibing S., Nataraj N.B., Sahay S., Schneider M., Wörner A., Becki C., Ishaque N., Feuerbach L., Michels B.E., Heßling B., Helm D., Will R., Yarden Y., Müller‐Decker K., Körner C., & Wiemann S. (2022). 5’isomiR-183-5p|+2 Elicits Tumor Suppressor Activity in a Negative Feedback Loop with E2F1.

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