Direct qtm system

Ethanol (96% v/v), methanol, HPLC-grade acetonitrile, phosphoric acid (85% w/v), and sodium chloride were obtained from Carlo Erba (Milan, Italy). HPLC-grade formic acid, hydrochloric acid (37% w/v), Type VI-porcine pancreatic α-amylase, pepsin from porcine gastric mucosa (≥400 U mg⁻¹), bile extract porcine, pancreatin (8 × USP) from porcine pancreas, sodium taurocholate (NaTC), sodium glycocholate (NaGC), sodium taurodeoxycholate (NaTCDC), sodium glycochenoxycholate (NaGCDC), protease from Streptomyces griseus type XIV (≥3.5 U mg⁻¹), viscozyme L cellulolytic enzyme mixture, sodium azide, and cholesterolamine were provided by Merck KGaA (Darmstadt, Germany).
Water was obtained from a Millipore Direct-QTM system (Merck-Millipore, Milan, Italy).
Pullulan gel filtration chromatography standard kit was purchased from Waters Corporation (Milford, MA, USA).

Moradyn corn cobs (Zea mays L. cv Moradyn), camelina cake (Camelina sativa L. Crantz), common bean seeds (Phaseolus vulgaris L.), and Arabic gum were kindly provided by FlaNat Research s.r.l. (Milano, Italy).
Ethanol (96% v/v), magnesium chloride, sodium chloride, calcium hydroxide, HPLC-grade formic acid, methanol, and acetonitrile were purchased from Carlo Erba (Milan, Italy). Water was obtained from a Millipore Direct-QTM system (Merck-Millipore, Milan, Italy). Cyanidin-3-O-glucoside (C3G) was purchased from Extrashynthese (Genay, France).